Establishing and culturing cell lines stably expressing cloned membrane proteins

Flow cytometry

HPLC with electrochemical or fluorescence detection of neurotransmitters

Electrophysiology (patch-clamp)

Molecular Biology (site-directed mutagenesis, Western Blotting)

Stereotaxic injections

Receptor binding techniques

Slice autoradiography

Subregional dissection of the human brain