- Two-photon laser scanning microscopy to assess Ca2+ - gradients in spinal dorsal horn neurons in vivo. -more-
- Labeling of lamina I projection neurons by injection of the retrograde tracer DiI into the rat brain stem.
- Extracellular single cell and field potential recording in spinal dorsal horn.
- Animal models of neuropathic and inflammatory pain
In vitro techniques - Spinal cord slice preparation, with long dorsal root attached, from rats and transgenic mice
- Whole-cell patch-clamp recordings under infrared differential interference contrast (IR-DIC) video microscopy to study intrinsic properties of nociceptive neurons and synaptic plasticity in superficial spinal dorsal horn
- Perforated whole-cell patch-clamp measurements in spinal cord slice preparations
- Microfluorometric measurement of [Ca2+]i with 2-Photon-Laserscanning microscopy and conventional video microscopy
- Dorsal root ganglion (DRG) preparation
Electrophysiological recordings from DRG neurons with sharp electrodes
Immunohistochemistry and Western blot
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