Characterisation of protein fractions of Jurkat cells applicable for proteome profiling experiments. Here, the 2D-PAGE spot pattern of isolated sub-cellular protein fractions such as mitochondria (annotated in red), nuclei (green) and cytosol (blue) are compared to cytoplasm (blue-red) and a total cell fraction (blue-red-green). SwissProt accession numbers of identified proteins are annotated in the appropriate gel sections and protein names listed in Tab. 1. Colour-coded circles indicate the position of the respective proteins in all fractions.
Overview
Whole 2D gel of cytoplasmic proteins, the gel section analysed in more detail is shown.
Section
Gel section of a total cell fraction. The position of several identified mitochondrial (red), nuclear (green) and cytosolic (blue) proteins is indicated. Proteins encircled in yellow are obviously under-represented in the total cell fraction.
Cytosol
Gel section of a cytosolic protein fraction.
Cytoplasm
Gel section of a cytoplasmic protein fraction. Note that essentially all protein spots detectable in C are also detectable in D, while several proteins spots detectable in D, amongst them the indicated mitochondrial proteins, are hardly or not detectable in C. On the other hand, several annotated proteins marked by yellow circles are almost undetectable in B and thus obviously under-represented.
Nuclei
Gel section of a nuclear matrix fraction. Note that the protein pattern is dramatically different from the other fractions and even the highly-abundant cytosolic proteins are hardly detectable.
Mitochondria
Gel section of a mitochondrial protein fraction. Again, the protein pattern is dramatically different from the other fractions and even the highly-abundant cytosolic proteins are hardly detectable.
