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NEWS (26 August 2005):
  • HN-NonLin has been updated to V1.1. Changes include:
  • Added support for triplicate tests in the Tools section.
  • Removed some rarley needed transformations and reorganized the Tools section.
  • Added an input field for the test date.
  • Some minor bufixes.
  • NonLin officially left Beta state.
  • Download HN-NonLin 1.1

NEWS (19 October 2004):
  • Some of the CELISA test kits recently (2004) shipped by CELLABS seem to be considerably more sensitive than previous batches. This may lead to very high backgrounds (and therefore inadequate dose reponse curves) in the drug sensitivity ELISA when testing at 0.05% parasite density. Solution: simply dilute the culture samples after culture but before performing the CELISA or use the new in-house ELISA at the original concentration (please see below). If you have any questions: harald.noedl@univie.ac.at.
  • Updated FAQ and SOP pages.

NEWS (15 July 2004):
  • New SOPs for the in-house ELISA available for download from the SOP page.
  • The Draft SOPs for a new, cheaper in-house ELISA based on 2 commercially available anti-HRP2 monoclonals are available for download. Although very convenient, the use of commercial ELISA test kits also made the HRP2 assay relatively expensive. We have therefore developed the SOPs for a simple double-site sandwich ELISA based on commercially available monoclonals (from ICLLAB) that will cut the price of the ELISA by around 80%. If you have any questions related to the new ELISA please send me an email at harald.noedl@univie.ac.at.


NEWS (29 March 2004):
  • New SOPs for the HRP2 field test available for download from the SOP page.
  • The field validation of the HRP2 assay against the WHO microtest in fresh field isolates was completed in 2003. The HRP2 field assay uses a procedure that further simplifies the handling and culturing of malaria parasites in the field by omitting centrifugation, washing, the use of serum, and dilution with uninfected red blood cells. The culture procedure is therefore similar to the WHO microtest (except for the fact that it uses 72 hours of incubation), whereas the reading of the results is done using a simple ELISA plate reader. The results of the HRP2 field test very closely matched those from the WHO microtest (R=0.98; P<0.001; mean log difference at IC50: 0.054)
  • We are currently finalising the SOPs for a new, cheaper in-house ELISA. Although very convenient, the use of ELISA test kits also made the HRP2 assay relatively expensive. We have developed the SOPs for a simple double-site sandwich ELISA based on commercially available monoclonals that will cut the price of the ELISA by around 80%. If you are interested to learn more about the new ELISA please send me an email at harald.noedl@univie.ac.at. SOPs will be available soon from this page.


Welcome to malaria.farch.net. This web site is dedicated to malaria drug resistance in general and to a novel experimental approach to drug sensitivity testing in particular. The rationale behind this website is to provide researchers with all information required for in vitro drug susceptibility testing and to provide extended information on a new P. falciparum drug sensitivity assay based on histidine rich protein II (HRP2), which - especially due to its simplicity - may have the potential to supplement or even replace traditional techniques. The HRP2 assay was published in Antimicrobial Agents and Cheotherapy: Noedl H., Wernsdorfer WH, Miller RS, Wongsrichanalai C. Histidine rich protein II, a novel approach to antimalarial drug susceptibility testing. Antimicrob Agents Chemother 2002 Jun; 46(6): 1658-64.

Although this website is primarily committed to the new assay, we hope that in the future these pages will become an ever evolving source of information on all subjects related to malaria drug sensitivity and its assessment. All researchers working in the field of malaria drug resistance are therefore very welcome to contribute to this website.

The new drug sensitivty assay, based on the measurement of Plasmodium falciparum histidine rich protein II (PfHRP2) in malaria culture, was developed at the Department of Immunology and Medicine, AFRIMS, Bangkok. The aim was to provide a simple, objective, easily reproducible test system, which includes the advantages of the currently used techniques, while omitting most of their disadvantages. During field validation in 2003 the new assay proved to be very sensitive (down to parasite densities of around 0.01%), and particularly simple to establish and perform, even in a temporary field lab.

Apart from standard operating procedures (SOPs), a frequently asked questions (FAQ) section and a discussion forum, the website includes the download of free software for the analysis of data obtained from the new assay as well as from conventional in vitro tests. In addition a section is dedicated to malaria drug resistance in general, as well as to the most commonly used traditional methods of assessing drug sensitivity. If you have any questions please feel free to contact me at harald.noedl@univie.ac.at.


H. Noedl
http://malaria.farch.net