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The HRP2 Assay
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Welcome to malaria.farch.net. This web site is dedicated to malaria drug resistance in general and to a novel experimental approach to drug sensitivity testing in particular. The rationale behind this website is to provide researchers with all information required for in vitro drug susceptibility testing and to provide extended information on a new P. falciparum drug sensitivity assay based on histidine rich protein II (HRP2), which - especially due to its simplicity - may have the potential to supplement or even replace traditional techniques. The HRP2 assay was published in Antimicrobial Agents and Cheotherapy: Noedl H., Wernsdorfer WH, Miller RS, Wongsrichanalai C. Histidine rich protein II, a novel approach to antimalarial drug susceptibility testing. Antimicrob Agents Chemother 2002 Jun; 46(6): 1658-64.
Although this website is primarily committed to the new assay, we hope that in the future these pages will become an ever evolving source of information on all subjects related to malaria drug sensitivity and its assessment. All researchers working in the field of malaria drug resistance are therefore very welcome to contribute to this website.
The new drug sensitivty assay, based on the measurement of Plasmodium falciparum histidine rich protein II (PfHRP2) in malaria culture, was developed at the Department of Immunology and Medicine, AFRIMS, Bangkok. The aim was to provide a simple, objective, easily reproducible test system, which includes the advantages of the currently used techniques, while omitting most of their disadvantages. During field validation in 2003 the new assay proved to be very sensitive (down to parasite densities of around 0.01%), and particularly simple to establish and perform, even in a temporary field lab.
Apart from standard operating procedures (SOPs), a frequently asked questions (FAQ) section and a discussion forum, the website includes the download of free software for the analysis of data obtained from the new assay as well as from conventional in vitro tests. In addition a section is dedicated to malaria drug resistance in general, as well as to the most commonly used traditional methods of assessing drug sensitivity. If you have any questions please feel free to contact me at firstname.lastname@example.org.