b-Galactosidase Assay with CPRG

 

 

 

 

Lysis Buffer:

0.25M Tris/HCl pH 7.4 (or better 8.0)

0.25% (v/v) NP40

2.5 mM EDTA

 

CPRG-substrate solution: 1 mg/ml (= 1.65 mM)

in PBS + 10 mM KCl, + 1 mM MgCl2

alternative substrate buffer:

60 mM Na2HPO4 pH 8.0, 1 mM MgCl2, 10 mM KCl, 50 mM Mercapto-ethanol

 

Stop solution: 0.5M Na­2CO3