Research Group of J. Schmid

For Scientists


(click images for further hyperlinks) 

from Schmid & Birbach
Cytokine Growth Factor Rev. (2008), Vol. 19, p157-65

Overview of projects and research interests

Current research interests focus on inflammation, thrombosis and cancer. Signal transduction pathways of inflammation are studied with special focus on the NF-kappa B signaling, as well as interconnections with other signaling processes in vascular biology and cancer .  

Link to the current special research program (SFB-F54)funded by the FWF:
Cellular mediators linking inflammation and thrombosis

Link to the last FWF-Project:
Cooperativity of Transcription Factors in Inflammation and Cancer

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projection of a FRET z-stack

Signal transduction networks are studied with a variety of experimental systems, such as cell culture of primary and transformed cells or transgene mouse models. Transfection and viral transduction strategies are applied to achieve either ectopic expression or gene suppression of effector molecules followed by analyzing a variety of biological readouts such as cell proliferation, apoptosis or activation of cells.  






Moreover, we investigate the dynamics of signaling molecules (for instance nucleocytoplasmic shuttling) in living cells using fluorescent protein chimeras and various high-end laser scanning microscopy techniques (such as fluorescence loss in photobleaching, FLIP, or fluorescence recovery after photobleaching, FRAP).  

example for FRAP analysis


FRET image
Interactions between proteins are studied by fluorescence resonance energy transfer (FRET) microscopy and verified with classical biochemical methods such as co-immunoprecipitation.  

FRET techniques are also applied to study protein-DNA interactions, as they occur when transcription factors bind to DNA

Spectral imaging is used to differentiate between overlapping fluorophores

unmixed GFP / YFP images
Besides these analyses of experimental models, we also investigate patient tissue samples using tissue arrays and novel methods of quantitative tissue cytometry to study correlations between candidate effector molecules in cancer development.  
human tissue sample
We establish transgenic mouse systems for the study of inflammation and cancer. Pro-malignant factors are combined with molecules of inflammatory reactions in a cooperative manner using organ-specific, inducible Cre recombinase approaches.

Some former Projects

J. A. Schmid, L. Mach, E. Paschke and J. Glössl: Accumulation of sialic acid in endocytic compartments interferes with the formation of mature lysosomes. J. Biol. Chem. 274 (27), 19063-71 (1999)
J. Exp. Med. (1998), 188, p211-6.

E. Piddini, J.A. Schmid (equally contributing first author), R. de Martin and C. Dotti: The Ras-like GTPase Gem is involved in cell shape remodelling and interacts with the novel kinesin-like protein KIF9. EMBO J. 20(15), 4076-4087 (2001).


 Johannes A. Schmid