Research Group of

Johannes Schmid

Home
Projects
Publications
Team
Lectures
For Scientists
Links
Positions
Calendar

Projects

Notes for potential reviewers of grant applications:
The funding situation in Austria is quite tight; the budget of the Austrian Science Fund (FWF), the major funding source for basic research, is only 23.8 € per resident, whereas this number is about twice as high in UK and nearly four times as high in Switzerland. Due to this financial situation, a proposed project has a significant chance for funding just in case of a high ranking. So even if the proposal gets a very good review it might not be funded if the score is below 90%. If a reviewer thinks that one of our proposed projects should be carried out, it will be necessary to rate it accordingly. This statement does not intend to put any pressure on the reviewers and we are certainly willing to accept any constructive feedback.
 

Examples of research projects (click images for further hyperlinks) 


from Schmid & Birbach
Cytokine Growth Factor Rev. (2008), Vol. 19, p157-65

Current research interests focus on inflammation, thrombosis and cancer. Signal transduction pathways of inflammation are studied with special focus on the NF-kappa B signaling, as well as interconnections with other signaling processes in vascular biology and cancer .  


Link to the current special research program (SFB-F54)funded by the FWF:
Cellular mediators linking inflammation and thrombosis

Link to the last FWF-Project:
Cooperativity of Transcription Factors in Inflammation and Cancer

projection of a FRET z-stack



Signal transduction networks are studied with a variety of experimental systems, such as cell culture of primary and transformed cells or transgene mouse models. Transfection and viral transduction strategies are applied to achieve either ectopic expression or gene suppression of effector molecules followed by analyzing a variety of biological readouts such as cell proliferation, apoptosis or activation of cells.  

 

 

 

 

GFP
 

Moreover, we investigate the dynamics of signaling molecules (for instance nucleocytoplasmic shuttling) in living cells using fluorescent protein chimeras and various high-end laser scanning microscopy techniques (such as fluorescence loss in photobleaching, FLIP, or fluorescence recovery after photobleaching, FRAP).  

example for FRAP analysis

 


FRET image
Interactions between proteins are studied by fluorescence resonance energy transfer (FRET) microscopy and verified with classical biochemical methods such as co-immunoprecipitation.  

co-IP
 
FRET techniques are also applied to study protein-DNA interactions, as they occur when transcription factors bind to DNA

Spectral imaging is used to differentiate between overlapping fluorophores


unmixed GFP / YFP images
Besides these analyses of experimental models, we also investigate patient tissue samples using tissue arrays and novel methods of quantitative tissue cytometry to study correlations between candidate effector molecules in cancer development.  
human tissue sample
We establish transgenic mouse systems for the study of inflammation and cancer. Pro-malignant factors are combined with molecules of inflammatory reactions in a cooperative manner using organ-specific, inducible Cre recombinase approaches.
 

Some former Projects

 
 
J. A. Schmid, L. Mach, E. Paschke and J. Glössl: Accumulation of sialic acid in endocytic compartments interferes with the formation of mature lysosomes. J. Biol. Chem. 274 (27), 19063-71 (1999)
J. Exp. Med. (1998), 188, p211-6.
 

E. Piddini, J.A. Schmid (equally contributing first author), R. de Martin and C. Dotti: The Ras-like GTPase Gem is involved in cell shape remodelling and interacts with the novel kinesin-like protein KIF9. EMBO J. 20(15), 4076-4087 (2001).

 

 Johannes A. Schmid