•  Collection of standard methods (from Hannah Neumeier's diploma thesis)
  
  
•  beta-Galactosidase CPRG plate reader assay
•  Cell cycle analysis by PI-staining and flow cytometry 
•  Co-immunoprecipitation
•  EMSA's (electrophoretic mobility shift assays)
•  SNT (In Situ Nick Translation for the morphological detection of apoptotic cells)
•  JC1-staining to detect apoptotic cells (via the mitochondrial membrane potential)
•  Kinase assays
•  Northern Blots
•  PCR-cloning
•  SDS-PAGE buffer recipes
•  Transfections with Calcium
•  Transfections with Lipofectamine Plus
•  Western Blots (for detection of myc, HA, flag, I-kappa B, GFP, TAK1)
•  Yeast-DNA preparation
•  Yeast transformation (link to Gietz lab)
  
  

MS-Excel Templates

•  DNA-calculations
•  Biorad Protein Assay
•  BCA-Protein Assay (Harald Freudenthaler)
•  FLIP (fluorescence loss in photobleaching)
•  FRAP (fluorescence recovery after photobleaching)
  - template for single values
  - template for single values + curve fitting ....NEW
    (Solver Add-In has to be activated, Macros enabled)
  - template for 5 replicates
  - template for single values with control region
•  FRET: correction factors, Youvan and Xia values
•  Molarity calculations
•  realtime PCR (quantitative PCR, qPCR)
  - with dilution curve to determine PCR effciency
•  realtime PCR with quantification of the PCR efficiency based on the amplification curve
•  SDS-PAGE: MW-calculations

Links to some of our research material:

•  DNA-constructs
•  Primers
•  Cell lines and stable transfectants

Link to the Belgian collection of
plasmids and microorganisms

Link to GoogleDocs: http://docs.google.com/ : log in with username: schmidgroup@googlemail.com and password

Some other Links:

•  Java-Application for calculation of molarities

Example Screenshot