• Collection of standard methods (from Hannah Neumeier's diploma thesis)
  
  
• beta-Galactosidase CPRG plate reader assay
• Cell cycle analysis by PI-staining and flow cytometry 
• Co-immunoprecipitation
• EMSA's (electrophoretic mobility shift assays)
• ISNT (In Situ Nick Translation for the morphological detection of apoptotic cells)
• JC1-staining to detect apoptotic cells (via the mitochondrial membrane potential)
• Kinase assays
• Northern Blots
• PCR-cloning
• SDS-PAGE buffer recipes
• Transfections with Calcium
• Transfections with Lipofectamine Plus
• Western Blots (for detection of myc, HA, flag, I-kappa B, GFP, TAK1)
• Yeast-DNA preparation
• Yeast transformation (link to Gietz lab)
  
  

MS-Excel Templates

• DNA-calculations
• Biorad Protein Assay
• BCA-Protein Assay (Harald Freudenthaler)
• FLIP (fluorescence loss in photobleaching)
• FRAP (fluorescence recovery after photobleaching)
• FRET: correction factors, Youvan and Xia values
• Molarity calculations
• realtime PCR (quantitative PCR, qPCR)
• SDS-PAGE: MW-calculations

Links to some of our research material:

•  DNA-constructs
•  Primers
•  Cell lines and stable transfectants

Link to GoogleDocs: http://docs.google.com/ : log in with username: schmidgroup@googlemail.com and password

Some other Links:

• Java-Application for calculation of molarities

Example Screenshot