Research Group of Johannes A. Schmid
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Scientific Articles:


Moser B, Hochreiter B, Herbst R, Schmid JA. Fluorescence colocalization microscopy analysis can be improved by combining object-recognition with pixel-intensity-correlation. Biotechnol J. 2017 Jan;12(1). doi: 10.1002/biot.201600332.


Kuttke M, Sahin E, Pisoni J, Percig S, Vogel A, Kraemmer D, Hanzl L, Brunner JS, Paar H, Soukup K, Halfmann A, Dohnal AM, Steiner CW, Blüml S, Basilio J, Hochreiter B, Salzmann M, Hoesel B, Lametschwandtner G, Eferl R, Schmid JA, Schabbauer G.

Myeloid PTEN deficiency impairs tumor-immune surveillance via immune-checkpoint inhibition.

Oncoimmunology. 2016 Mar 28;5(7):e1164918. doi: 10.1080/2162402X.2016.1164918.

61 Schmid JA. Endoplasmosis and exoplasmosis: the evolutionary principles underlying endocytosis, exocytosis, and vesicular transport. Wien Med Wochenschr. 2016 May;166(7-8):236-241.

Puujalka E, Heinz M, Hoesel B, Friedl P, Schweighofer B, Wenzina J, Pirker C, Schmid JA, Loewe R, Wagner EF, Berger W, Petzelbauer P. Opposing Roles of JNK and p38 in Lymphangiogenesis in Melanoma. J Invest Dermatol. 2016 May;136(5):967-77


Hoesel B, Malkani N, Hochreiter B, Basílio J, Sughra K, Ilyas M, Schmid JA. Sequence-function correlations and dynamics of ERG isoforms. ERG8 is the black sheep of the family. Biochim Biophys Acta. 2016 Feb;1863(2):205-18.

Bernhard Hochreiter, Alan Pardo Garcia and Johannes A. Schmid. Fluorescent Proteins as Genetically Encoded FRET Biosensors in Life Sciences. Sensors (2015), 15(10), 26281-26314; doi:10.3390/s151026281

Kreiseder B, Holper-Schichl YM, Muellauer B, Jacobi N, Pretsch A, Schmid JA, Martin R, Hundsberger H, Eger A, Wiesner C. Alpha-Catulin Contributes to Drug-Resistance of Melanoma by Activating NF-κB and AP-1. PLoS One. (2015) Mar 20;10(3):e0119402. doi: 10.1371/journal.pone.0119402. eCollection 2015.


Kunze M, Malkani N, Maurer-Stroh S, Wiesinger C, Schmid JA, Berger J. Mechanistic Insights into PTS2-mediated Peroxisomal Protein Import: The Co-receptor PEX5L drastically increases the Interaction Strength between the Cargo Protein and the Receptor PEX7. J Biol Chem. (2015) Feb 20;290(8):4928-40.


Forster F, Paster W, Supper V, Schatzlmaier P, Sunzenauer S, Ostler N, Saliba A, Eckerstorfer P, Britzen-Laurent N, Schütz G, Schmid JA, Zlabinger GJ, Naschberger E, Stürzl M, Stockinger H.: Guanylate binding protein 1-mediated interaction of T cell antigen receptor signaling with the cytoskeleton. J Immunol. (2014) Jan 15;192(2):771-81.


Luiskandl S., Woller B., Schlauf M., Schmid J.A., Herbst R.:
Endosomal trafficking of the receptor tyrosine kinase MuSK proceeds via clathrin-dependent pathways, Arf6 and actin. FEBS J. (2013), 280(14):3281-3297.


Hoesel B. and Schmid J.A#.: The complexity of NF-kappaB signaling in inflammation and cancer. Molecular Cancer (2013), 12(1):86.


Asare Y., Shagdarsuren E., Schmid J.A., Tilstam P.V., Grommes J., El Bounkari O., Schutz A.K., Weber C., de Winther M.P., Noels H. and Bernhagen J.: Endothelial CSN5 impairs NF-kappa B activation and monocyte adhesion to endothelial cells and is highly expressed in human atherosclerotic lesions. Thromb Haemost (2013), 110(1):141-152.


Haschemi, A., Kosma, P., Gille, L., Evans, C.R., Burant, C.F., Starkl, P., Knapp, B., Haas, R., Schmid, J.A., Jandl, C., Amir, S., Lubec, G., Park, J., Esterbauer, H., Bilban, M., Brizuela, L., Pospisilik, J.A., Otterbein, L.E., and Wagner, O., The Sedoheptulose Kinase CARKL Directs Macrophage Polarization through Control of Glucose Metabolism. Cell Metab (2012). 15(6): 813-26.


Ginter, T., Bier, C., Knauer, S.K., Sughra, K., Hildebrand, D., Munz, T., Liebe, T., Heller, R., Henke, A., Stauber, R.H., Reichardt, W., Schmid, J.A., Kubatzky, K.F., Heinzel, T., and Kramer, O.H., Histone deacetylase inhibitors block IFNgamma-induced STAT1 phosphorylation. Cell. Signal. (2012), 24:1453-1460.


Skvara H, Plut U, Schmid JA, Jonak C.: Combining in vivo reflectance with fluorescence confocal microscopy provides additive information on skin morphology. Dermatol Pract Concept. (2012) Jan 31;2(1):3-12.


Kunze, M., Neuberger, G., Maurer-Stroh, S., Ma, J., Eck, T., Braverman, N., Schmid, J.A., Eisenhaber, F., and Berger, J., Structural requirements for interaction of peroxisomal targeting signal 2 and its receptor PEX7J. Biol. Chem. (2011), 286:45048-45062.


Jonak, C., Skvara, H., Kunstfeld, R., Trautinger, F. and Schmid, J.A. Intradermal Indocyanine Green for In Vivo Fluorescence Laser Scanning Microscopy of Human Skin: A Pilot Study. PLoS ONE (2011). 6(8):e23972.


Skvara, H., Kittler, H., Schmid, J.A. and Jonak, C. In vivo fluorescence confocal microscopy: Indocyanine green enhances the contrast of epidermal and dermal structures. J. Biomed. Optics (2011) Sep;16(9):096010.


Birbach, A., Eisenbarth, D., Kozakowski, N., Ladenhauf, E., Schmidt-Supprian, M. and Schmid, J.A. Persistent Inflammation Leads to Proliferative Neoplasia and Loss of Smooth Muscle Cells in a Prostate Tumor Model. Neoplasia (2011). Aug;13(8):692-703.


Malkani, N., and Schmid J.A. Some secrets of fluorescent proteins: distinct bleaching in various mounting fluids and photoactivation of cyan fluorescent proteins at YFP-excitation. PLoS ONE (2011). 6:e18586.


Sughra, K., A. Birbach, R. de Martin, and Schmid J.A.. Interaction of the TNFR-Receptor Associated Factor TRAF1 with I-Kappa B Kinase-2 and TRAF2 Indicates a Regulatory Function for NF-Kappa B Signaling. PLoS ONE (2010). 5:e12683.


Jiang M., Strand D.W., Fernandez S., He Y., Yi Y., Birbach A., Qiu Q., Schmid J., Tang D.G. and Hayward S.W. Functional remodeling of benign human prostatic tissues in vivo by spontaneously immortalized progenitor and intermediate cells. Stem Cells. (2010) Feb;28(2):344-56.


Orel, L., H. Neumeier, K. Hochrainer, B.R. Binder, and J.A. Schmid. Crosstalk between the NF-κB activating IKK-complex and the CSN signalosome. Journal of Cellular and Molecular Medicine (2010). 14:1555-1568.


Birbach, A., Casanova, E. and Schmid, J.A.: A Probasin-MerCreMer BAC allows inducible recombination in the mouse prostate. Genesis (2009) 47(11):757-64.


Zebedin, E., Simma, O., Schuster, C., Putz, E. M., Fajmann, S., Warsch, W., Eckelhart, E., Stoiber, D., Weisz, E., Schmid, J. A., Pickl, W. F., Baumgartner, C., Valent, P., Piekorz, R. P., Freissmuth, M. and Sexl, V.: Leukemic challenge unmasks a requirement for PI3K{delta} in NK-cell-mediated tumor surveillance. Blood. (2008), 112:4655-64.


Korkhov, V. M., Milan-Lobo, L., Zuber, B., Farhan, H., Schmid, J. A., Freissmuth, M. and Sitte, H. H.: Peptide-based interactions with calnexin target misassembled membrane proteins into endoplasmic reticulum-derived multilamellar bodies. J Mol Biol. (2008), Vol. 378, p337-52.


Wiesner, C., Winsauer, G., Resch, U., Hoeth, M., Schmid, J. A., van Hengel, J., van Roy, F., Binder, B. R. and de Martin, R.: Alpha-catulin, a Rho signalling component, can regulate NF-kappaB through binding to IKK-beta, and confers resistance to apoptosis. Oncogene. (2008), Vol. 27, p2159-69.


Schmid, J. A.# and Birbach, A.: IkappaB kinase beta (IKKbeta/IKK2/IKBKB)--a key molecule in signaling to the transcription factor NF-kappaB. Cytokine Growth Factor Rev. (2008), Vol. 19, p157-65.


Farhan, H., Reiterer, V., Korkhov, V. M., Schmid, J. A., Freissmuth, M. and Sitte, H. H.: Concentrative export from the endoplasmic reticulum of the gamma-aminobutyric acid transporter 1 requires binding to SEC24D. J Biol Chem. (2007), Vol. 282, p7679-89.


Schmid, J. A.# and Birbach, A.: Fluorescent proteins and fluorescence resonance energy transfer (FRET) as tools in signaling research. Thromb Haemost. (2007), Vol. 97, p378-384.


Schuster, I., Egger, H., Herzig, G., Reddy, G. S., Schmid, J. A., Schussler, M. and Vorisek, G.: Selective inhibitors of vitamin D metabolism--new concepts and perspectives. Anticancer Res. (2006), Vol. 26, p2653-68.


Yang, J., Amiri, K. I., Burke, J. R., Schmid, J. A. and Richmond, A.: BMS-345541 targets inhibitor of kappaB kinase and induces apoptosis in melanoma: involvement of nuclear factor kappaB and mitochondria pathways. Clin Cancer Res. (2006), Vol. 12, p950-60.


Loscher, M., Fortschegger, K., Ritter, G., Wostry, M., Voglauer, R., Schmid, J. A., Watters, S., Rivett, A. J., Ajuh, P., Lamond, A. I., Katinger, H. and Grillari, J.: Interaction of U-box E3 ligase SNEV with PSMB4, the beta7 subunit of the 20 S proteasome. Biochem J. (2005), Vol. 388, p593-603.


Schmid, J. A.# and Neumeier, H.: Evolutions in science triggered by green fluorescent protein (GFP). Chembiochem. (2005), Vol. 6, p1149-56.


Ecker, R. C., de Martin, R., Steiner, G. E. and Schmid, J. A.: Application of spectral imaging microscopy in cytomics and fluorescence resonance energy transfer (FRET) analysis. Cytometry A. (2004), Vol. 59, p172-81.


Birbach, A., Bailey, S. T., Ghosh, S. and Schmid, J. A.#: Cytosolic, nuclear and nucleolar localization signals determine subcellular distribution and activity of the NF-kappaB inducing kinase NIK. J Cell Sci. (2004), Vol. 117, p3615-24.


Just, H., Sitte, H. H., Schmid, J. A., Freissmuth, M. and Kudlacek, O.: Identification of an additional interaction domain in transmembrane domains 11 and 12 that supports oligomer formation in the human serotonin transporter. J Biol Chem. (2004), Vol. 279, p6650-7.


Hofer-Warbinek, R., Schmid, J. A., Mayer, H., Winsauer, G., Orel, L., Mueller, B., Wiesner, C., Binder, B. R. and de Martin, R.: A highly conserved proapoptotic gene, IKIP, located next to the APAF1 gene locus, is regulated by p53. Cell Death Differ. (2004), Vol. 11, p1317-25.


Spahn, L., Siligan, C., Bachmaier, R., Schmid, J. A., Aryee, D. N. and Kovar, H.: Homotypic and heterotypic interactions of EWS, FLI1 and their oncogenic fusion protein. Oncogene. (2003), Vol. 22, p6819-29.


Baak, J. P., Path, F. R., Hermsen, M. A., Meijer, G., Schmid, J. and Janssen, E. A.: Genomics and proteomics in cancer. Eur J Cancer. (2003), Vol. 39, p1199-215.


Ebner, K., Bandion, A., Binder, B. R., de Martin, R. and Schmid, J. A.#: GMCSF activates NF-kappaB via direct interaction of the GMCSF receptor with IkappaB kinase beta. Blood. (2003), Vol. 102, p192-9.


Schmid, J. A.# and Sitte, H. H.: Fluorescence resonance energy transfer in the study of cancer pathways. Curr Opin Oncol. (2003), Vol. 15, p55-64.


Henkler, F., Baumann, B., Fotin-Mleczek, M., Weingartner, M., Schwenzer, R., Peters, N., Graness, A., Wirth, T., Scheurich, P., Schmid, J. A. and Wajant, H.: Caspase-mediated cleavage converts the tumor necrosis factor (TNF) receptor-associated factor (TRAF)-1 from a selective modulator of TNF receptor signaling to a general inhibitor of NF-kappaB activation. J Biol Chem. (2003), Vol. 278, p29216-30.


Schmid, J. A.#: Cover the eyes of Lady Justice. EMBO Rep. (2003), Vol. 4, p734-6.


Gruber, F., Hufnagl, P., Hofer-Warbinek, R., Schmid, J. A., Breuss, J. M., Huber-Beckmann, R., Lucerna, M., Papac, N., Harant, H., Lindley, I., de Martin, R. and Binder, B. R.: Direct binding of Nur77/NAK-1 to the plasminogen activator inhibitor 1 (PAI-1) promoter regulates TNF alpha -induced PAI-1 expression. Blood. (2003), Vol. 101, p3042-8.


Birbach, A., Gold, P., Binder, B. R., Hofer, E., de Martin, R. and Schmid, J. A.#: Signaling molecules of the NF-kappa B pathway shuttle constitutively between cytoplasm and nucleus. J Biol Chem. (2002), Vol. 277, p10842-51.


Fotin-Mleczek, M., Henkler, F., Samel, D., Reichwein, M., Hausser, A., Parmryd, I., Scheurich, P., Schmid, J. A. and Wajant, H.: Apoptotic crosstalk of TNF receptors: TNF-R2-induces depletion of TRAF2 and IAP proteins and accelerates TNF-R1-dependent activation of caspase-8. J Cell Sci. (2002), Vol. 115, p2757-70.


Spahn, L., Petermann, R., Siligan, C., Schmid, J. A., Aryee, D. N. and Kovar, H.: Interaction of the EWS NH2 terminus with BARD1 links the Ewing's sarcoma gene to a common tumor suppressor pathway. Cancer Res. (2002), Vol. 62, p4583-7.


Schmid, J. A., Just, H. and Sitte, H. H.: Impact of oligomerization on the function of the human serotonin transporter. Biochem Soc Trans. (2001), Vol. 29, p732-6.


Oitzinger, W., Hofer-Warbinek, R., Schmid, J. A., Koshelnick, Y., Binder, B. R. and de Martin, R.: Adenovirus-mediated expression of a mutant IkappaB kinase 2 inhibits the response of endothelial cells to inflammatory stimuli. Blood. (2001), Vol. 97, p1611-7.


Schmid, J. A., Scholze, P., Kudlacek, O., Freissmuth, M., Singer, E. A. and Sitte, H. H.: Oligomerization of the human serotonin transporter and of the rat GABA transporter 1 visualized by fluorescence resonance energy transfer microscopy in living cells. J Biol Chem. (2001), Vol. 276, p3805-10.


Piddini, E., Schmid, J. A.+, de Martin, R. and Dotti, C. G.: The Ras-like GTPase Gem is involved in cell shape remodelling and interacts with the novel kinesin-like protein KIF9. Embo J. (2001), Vol. 20, p4076-87.


De Martin, R., Hoeth, M., Hofer-Warbinek, R. and Schmid, J. A.: The transcription factor NF-kappa B and the regulation of vascular cell function. Arterioscler Thromb Vasc Biol. (2000), Vol. 20, pE83-8.


Schmid, J. A.#, Birbach, A., Hofer-Warbinek, R., Pengg, M., Burner, U., Furtmuller, P. G., Binder, B. R. and de Martin, R.: Dynamics of NF kappa B and Ikappa Balpha studied with green fluorescent protein (GFP) fusion proteins. Investigation of GFP-p65 binding to DNa by fluorescence resonance energy transfer. J Biol Chem. (2000), Vol. 275, p17035-42.


Hofer-Warbinek, R., Schmid, J. A., Stehlik, C., Binder, B. R., Lipp, J. and de Martin, R.: Activation of NF-kappa B by XIAP, the X chromosome-linked inhibitor of apoptosis, in endothelial cells involves TAK1. J Biol Chem. (2000), Vol. 275, p22064-8.


de Martin, R., Schmid, J. A. and Hofer-Warbinek, R.: The NF-kappaB/Rel family of transcription factors in oncogenic transformation and apoptosis. Mutat Res. (1999), Vol. 437, p231-43.


Schmid, J. A.#, Mach, L., Paschke, E. and Glossl, J.: Accumulation of sialic acid in endocytic compartments interferes with the formation of mature lysosomes. Impaired proteolytic processing of cathepsin B in fibroblasts of patients with lysosomal sialic acid storage disease. J Biol Chem. (1999), Vol. 274, p19063-71.


Schmid, J. A.#, Ellinger, I. and Kosma, P.: In vitro fusion of tissue-derived endosomes and lysosomes. Eur J Cell Biol. (1998), Vol. 77, p166-74.


Stehlik, C., de Martin, R., Kumabashiri, I., Schmid, J. A., Binder, B. R. and Lipp, J.: Nuclear factor (NF)-kappaB-regulated X-chromosome-linked iap gene expression protects endothelial cells from tumor necrosis factor alpha-induced apoptosis. J Exp Med. (1998), Vol. 188, p211-6.


Ambros, P. F., Schmid, J., Rumpler, S., Binder, B. R. and de Martin, R.: Localization of the human I-kappaB kinase-beta (IKBKB) to chromosome 8p11.2 by fluorescence in situ hybridization and radiation hybrid mapping. Genomics. (1998), Vol. 54, p575-6.


Schmid, J. A.# and Billich, A.: Simple method for high sensitivity chemiluminescence ELISA using conventional laboratory equipment. Biotechniques. (1997), Vol. 22, p278-80.


Schmid, J. A.#: The acidic environment in endocytic compartments. Biochem J. (1994), Vol. 303 ( Pt 2), p679-80.


Murphy, R. F., Schmid, J. and Fuchs, R.: Endosome maturation: insights from somatic cell genetics and cell-free analysis. Biochem Soc Trans. (1993), Vol. 21 ( Pt 3), p716-20.


Book Reviews

- ChemBioChem
(2009), Vol. 17, 2801-2802: Review on: Viral Applications of Green Fluorescent Protein, Methods and Protocols; Methods in Molecular Biology, Vol. 515


  1. J. A. Schmid: Diplomarbeit (1990): Veränderte Prozessierung von Kathepsin B in Fibroblasten von Patienten mit Sialinsäure-Speicherkrankheit.

  2. J. A. Schmid: Dissertation (1994): Endocytose und Transcytose in Hepatocyten

Online Report for the European Advanced Light Microscopy Facility

Chapters in textbooks

J. A. Schmid, H. Klapper and R. Fuchs: Characterization of basolateral and apical endocytic pathways in rat hepatocytes. In: Hepatic Endocytosis of Lipids and Proteins, ed. by: E. Windler and H. Greten, p. 31-36, W. Zuckerschwerdt Verlag 1992. 


Johannes A. Schmid