Techniques

Techniques

Immunohistology, including immunofluorescence microscopy and Fluorescence in situ hybridization (FISH) of skin cryosections.

FACS (including intracellular cytokine staining).

Cell isolation from various organs by flow cytometry (up to single-cell sorting) or microbeads.

Cell culture and functional assays with low input cell numbers (including cytokine stimulation, proliferation assays, migration assays, cytotoxicity assays).

ELISA

Quantitative RT-PCR

Microbial DNA and RNA Extraction