Info Poster YSA 2021
Titel: A Novel Approach for Quantifying Chlamydia trachomatis Elementary Bodies and Reticulate Bodies by Flow Cytometry
Autoren: Romana Klasinc (1), Michael Reiter (1), Andreas Spittler (2), Hannes Stockinger (1)
(1) Institute for Hygiene and Applied Immunology, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna, Vienna, Austria
(2) Core Facility Flow Cytometry and Department of Surgery, Research Laboratories, Medical University of Vienna, Vienna, Austria
In this study the authors developed a highly reproducible flow cytometry-based method for quantification and viability assessment of Chlamydia trachomatis preparations. This novel approach enables a fast, cell culture-independent enumeration of C. trachomatis stocks and represents a valuable tool for planning subsequent down-stream experiments.
Vibrio cholerae (non O1/non O139) at Eastern Austrian bathing sites
Rehm C , Kolm C , Jakwerth S , Pleininger S , Heger F , Lippert K , Indra A , Hein T , Farnleitner AH , Reischer GH , Kirschner AKT
1 Karl Landsteiner University of Health Sciences, Division Water Quality and Health, Krems, Austria
2 Medical University of Vienna, Institute for Hygiene and Applied Immunology, Vienna, Austria
3 Interuniversity Cooperation Centre Water & Health; www.waterandhealth.at
4 Technische Universität Wien, Institute for Institute of Chemical, Environmental and Bioscience Engineering, Vienna, Austria
5 Austrian Agency for Health and Food Safety, (AGES), Institute for Medical Microbiology and Hygiene, Vienna, Austria
6 Paracelsus Medical University of Salzburg, Salzburg, Austria
7 University of Natural Resources and Life Sciences, Vienna (BOKU), Institute of Hydrobiology and Aquatic Ecosystem Management, Vienna, Austria
8 Wassercluster Lunz, Lunz, Austria
In this study we are going to determine the prevalence, abundance and distribution of Vibrio cholerae nonO1/nonO139 at selected bathing sites along spatiotemporal environmental gradients. Therefore we use newly developed “state of the art” methods to obtain solid quantitative data. By combining the obtained information with a large set of environmental parameters, we are going to design prediction models for the distribution, prevalence and abundance of Vibrio cholerae nonO1/nonO139 in bathing waters. The data will also fuel risk assessment models to assess the relevance of these pathogens for public health.
back to: Institute for Hygiene and Applied Immunology