address: CePII, Institute for Hygiene and Applied Immunology, Lazarettgasse 19, room G3.07, A-1090 Vienna, Austria
research interests: T cell antigen recognition, immunology, cell biology, signal transduction, imaging, protein engineering, biophysics, structural biology
In the Huppa lab we are interested in how the adaptive and innate branch of the immune system work together to distinguish between friend and foe. For the most part our curiosity revolves around two questions:
- How do lymphocytes, in particular T cells, recognize antigens on a molecular, subcellular and cellular level?
- How do Antigen Presenting Cells modulate antigen-specific T cell responses?
We are aiming for quantitative answers by cell biological, biophysical and genetic means to explain how T cells establish their exquisite antigen selectivity and sensitivity and maintain the right balance between tolerance and immunity.
To this end we engineer fluorescent probes and functionalized lipid bilayers, which we employ to study primary T cells and APCs in innovative laser microscopy experiments. We complement microscopy with state of the art methods in immunology and molecular biology to monitor and manipulate gene expression and immune function.
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education and research experience
- undergraduate studies of biochemistry at the Freie Universität Berlin, Germany
- graduate thesis work at the Massachusetts Institute of Technology
- postdoctoral fellow and research associate at Stanford University School of Medicine
(please contact Johannes Huppa for pdf-reprints)
- Huppa J.B. & Davis MM (2013) The interdisciplinary science of T-cell recognition. Advances in Immunology 119: 1-50
- Guy C.S., Vignali K.M., Temirov J., Bettini M.L., Overacre A.E., Smeltzer M., Zhang H., Huppa J.B., Tsai Y.H., Lobry C., Xie J., Dempsey P.J., Crawford H.C., Aifantis I., Davis .M., Vignali D.A. (2013) Distinct TCR signaling pathways drive proliferation and cytokine production in T cells. Nature Immunology 14: 262-270
- Axmann M., Huppa J.B., Davis M.M., Schütz G.J. (2012) Determination of interaction kinetics between the T cell receptor and peptide-loaded MHC class II via single-molecule diffusion measurements. Biophysical Journal 103 (2): L17-19
- Xie J., Huppa J.B., Newell E.W., Huang J., Ebert P.J., Li Q.J., Davis M.M. (2012) Photocrosslinkable pMHC monomers stain T cells specifically and cause ligand-bound TCRs to be 'preferentially' transported to the cSMAC. Nature Immunology 13:674-80
- Huppa J.B., Axmann, M., Mörtelmaier M.A., Lillemeier B.F., Newell E.W, Brameshuber M., Klein L.O., Schütz G.J., Davis M.M. (2010) TCR-peptide-MHC interactions in situ show accelerated kinetics and increased affinity. Nature 463: 963-7
- Lillemeier B.F., Mörtelmaier M.A., Forstner M.B., Huppa, J.B., Groves, J.T., Davis, M.M. (2010) TCR and LAT are expressed in separate membrane domains and concatenate during activation. Nature Immunology 11, 90-97
- Kuhns M.S., Girvin A.T., Klein L.O., Chen R., Jensen K.D., Newell E.W., Huppa J.B., Lillemeier B.F., Huse M., Chien Y.H., Garcia K.C., Davis M.M. (2010) Evidence for a functional sidedness to the ab TCR. Proceedings of the National Academy of Science (USA) 107: 5094-9
- Davis M.M., Krogsgaard M., Huse M., Huppa J., Lillemeier B.F. Li Q.J. (2007) T cells as self-referential, sensory organ. Annual Reviews in Immunology 25:681-95
- Huppa J.B., Davis M.M. (2003) T cell antigen recognition and the immunological synapse. Nature Reviews in Immunololgy 3:973-83
- Huppa J.B., Gleimer M., Sumen C., Davis M.M. (2003) Continuous T cell receptor signaling required for synapse maintenance and full effector potential. Nature Immunology 4:749-55
find all publications by Johannes Huppa in PubMed
download Johannes Huppa's CV here