Cardiovascular Diseases; Cardiovascular Physiological Processes; Cell Membrane Permeability; Intracellular Signaling Peptides and Proteins; Physiology; Serpins
My research focus are non-inhibitory functions of serine protease inhibitors (serpins), especially functions in cell signaling. We are focusing on serpinA5 (protein C inhibitor, PCI), a secreted protease inhibitor with wide tissue distribution and broad protease reactivity. SerpinA5 can be internalized by cells in a phospholipid-mediated process and then translocate to the nucleus. Based on these results we are currently investigating the mechanism of serpinA5-internalization. We are also working on the identification of intracellular/nuclear interaction partners and on the analysis of the biological relevance of these interactions.
Techniques, methods & infrastructure
Diverse basic molecular and cellular biology techniques, immunological and functional assays, subcellular fractionation, 2D-electrophoresis, flow cytometry, immunohistochemistry, confocal microscopy, analysis of protein-phospholipid interactions, phagocytosis assays, expression and purification of recombinant proteins, transgenic animals.