Cardiomyopathy, Hypertrophic; Infarction; Myocardial Ischemia; Myocardial Reperfusion Injury
Cardiovascular physiology, pathophysiology and pharmacology; experimental models of myocardial ischemia/reperfusion and infarction, cardiac arrhythmias, testing of newly developed antiischemic compounds. Focus on the endogenous adaptation of the heart against ischemic and reperfusion injury (e.g remote conditionings). Ischemia and reperfusion-induced release of reactive molecules and their downstream pathways, cardioprotective mechasnims relate to nitric oxide signaling pathway. The protective role of vagus stimulation on diseased heart and the possible cardioprotecvtive mechanism of non neuronal cholinerg system. Metabolic syndrome in vasculature and in myocardium. Left ventricle remodeling and heart failure, basic mechanism and clinical aspect.
Techniques, methods & infrastructure
In vivo: acute ischemia/reperfusion model of dog, pig and rat. Electrical stimulation of vagus nerve (rat, pig), diabetic model on rat. Minor experience working with mice with ischemia/reperfusion and LV remodelling. Myocardial infarction model on rats. Isolated working heart model on rat hearts. In vitro: Protein expression assay (Western blot), fluorescent staining, nitrate/nitrite assay, arginase activity, ELISA and histology. Assessment of vascular reactivity (myograph).