Effects of drugs on ion channels; Neuropharmacology; Neurophysiology; Patch-Clamp Techniques; Potassium Channels, Voltage-Gated
I am interested to understand the modulation of ion channels involved in controlling the excitability of peripheral neurons. Two ion channel families, that control excitability are the voltage-gated potassium channel family Kv7 and calcium-activated chloride channels. The activity of both ion channel families is controlled by Gq-coupled receptors via depletion of PIP2 and/ or increase in intracellular calcium. One one hand I want to investigate the endogenous modulation of these channels via G-protein coupled receptors for example in sensory neurons which helps us understand how inflammatory mediators affect nociception. On the other hand I investgate small molecule modulators of these ion channels which might prove useful as analgesics.
Techniques, methods & infrastructure
Primary neuronal cultures: of superior cervical ganglion (SCG) neurons, dorsal root ganglia (DRG) neurons, and dorsal horn cultures; Voltage- and current-clamp recordings;