Calcium Channels, L-Type; Epilepsy; Metabolism; Mitochondria; Neurons
My main research focus is to understand the proces of epileptogenesis and the role that neuronal metabolism plays in it. More specifically, we study how neuronal firing of various intensities affects mitochondrial ATP production and glycolysis.
Techniques, methods & infrastructure
We use primary cultures of mouse and rat hippocampal neurons. For the investigations of neuronal metabolism and calcium signaling we use neurons transiently expressing/stained with fluorescent-based indicators and imaging by laser-scanning confocal microscopy. Neuronal activity is assesed by minimally invasive perforated patch recordings using patch-clamp electrophysiology.