Keywords

Animal models; Endothelial Cells; Inflammation; Lymphatic System; Primary Cell Culture

Research interests

(1)     Determination of anti-inflammatory properties of plant- derived substances in in vitro and in vivo models

(2)     Identification of substances capable to counteract inflammation and senescence of endothelial cells, by utilizing endothelial cell cultures and live-cell imaging techniques (cooperation with Ao.Univ.-Prof. Dr. Johannes Breuss)

(3)     Analysis of the mechanism of development of lymphatic system and its role in metastatic spreading of cancer cells

Techniques, methods & infrastructure

Isolation, cultivation and characterization of primary endothelial cells, immunohistochemistry, flow cytometry, qPCR, ELISA, western blot, microscopy (within intensive cooperation with Ao.Univ.-Prof. Dr. Johannes Breuss) and work with gene-deficient mice.

Grants

• Metal complexes with dual anti-inflammatory activity (2021)
  Source of Funding: FWF (Austrian Science Fund), International project
  Principal Investigator
• Strategies for combating senescence in endothelial cells (2019)
  Source of Funding: FWF (Austrian Science Fund), Individual projects
  Principal Investigator

Selected publications

1. Uhrin, P. et al., 2018. Vascular smooth muscle cell proliferation as a therapeutic target. Part 2: Natural products inhibiting proliferation. Biotechnology Advances, 36(6), pp.1608–1621. Available at: http://dx.doi.org/10.1016/j.biotechadv.2018.04.002.
2. Brown, M. et al., 2018. Lymph node blood vessels provide exit routes for metastatic tumor cell dissemination in mice. Science, 359(6382), pp.1408–1411. Available at: http://dx.doi.org/10.1126/science.aal3662.
3. Khan, S.Y. et al., 2017. Premature senescence of endothelial cells upon chronic exposure to TNFα can be prevented by N-acetyl cysteine and plumericin. Scientific Reports, 7(1). Available at: http://dx.doi.org/10.1038/srep39501.
4. Uhrin, P. et al., 2010. Novel function for blood platelets and podoplanin in developmental separation of blood and lymphatic circulation. Blood, 115(19), pp.3997–4005. Available at: http://dx.doi.org/10.1182/blood-2009-04-216069.
5. Uhrin, P. et al., 2000. Disruption of the protein C inhibitor gene results in impaired spermatogenesis and male infertility. Journal of Clinical Investigation, 106(12), pp.1531–1539. Available at: http://dx.doi.org/10.1172/JCI10768.