Heat-labile Escherichia coli toxin enhances the induction of allergen-specific IgG antibodies in epicutaneous patch vaccination
C. R. Cabauatan1, R. Campana1, K. Niespodziana1, C. Reinisch2, U. Lundberg2, A. Meinke2, R. Henning3, A. Neubauer3 & R. Valenta1
1Division of Immunopathology, Department of Pathophysiology and Allergy Research, Center for Pathophysiology, Infectiology and Immunology, Medical University of Vienna
2Valneva Austria GmbH, Campus Vienna Biocenter
3Biomay AG, Vienna, Austria
Epicutaneous allergen-specific immunotherapy (EPIT) is proposed as an alternative route for allergen-specific immunotherapy. The patch delivery system (PDS) is one of the techniques in EPIT that simply involves light abrasion on the skin followed by the application of a patch with an antigen and an adjuvant.
In this study, we used recombinant major birch pollen allergen Bet v 1 as a model allergen. We compared epicutaneous administration of the allergen with and without heat-labile enterotoxin (LT) as adjuvant via PDS with the classical immunization based on subcutaneous injection of aluminum hydroxide-adsorbed rBet v 1 in outbred guinea pigs. We were able to show that subcutaneous immunization with aluminum hydroxide-adsorbed rBet v 1 and epicutaneous administration of rBet v 1 with LT induced allergen-specific IgG antibodies. Our most important result is that these induced IgG antibodies could block Bet v 1-specific IgE responses in sera from birch allergic patients. This suggests that patch vaccination with rBet v 1 in combination with LT may be a promising strategy for allergen-specific immunotherapy against birch pollen allergy.