MedUni Wien    Intranet    MedUni Wien - Shop    Universitätsbibliothek    Universitätsklinikum AKH Wien  
 
cepii_allergie.png
 
 
 
Hauptnavigation
  • Home
  • Allgemeine Informationen
  • Forschung
  • Studium & Lehre
  • FachärztInnenausbildung
 
IPA / Single View
 
Subnavigation

Research News

 

Generation of high affinity ICAM-1-specific nanobodies and evaluation of their suitability for allergy treatment

Ines Zettl, Tatiana Ivanova, Mohammed Zghaebi, Marina V. Rutovskaya, Isabella Ellinger, Oksana Goryainova, Jessica Kollárová, Sergio Villazala-Merino, Christian Lupinek, Christina Weichwald, Anja Drescher, Julia Eckl-Dorna,...[more]

 

Nothobranchius furzeri, the Turquoise Killifish: A Model of Age-Related Osteoporosis?

Maria Butylina, PhD-student in the group of Prof. Dr. Peter Pietschmann, published recently her research on Nothobranchius furzeri, the turquoise killifish, in Gerontology. [more]

 

Isolation of nanobodies with potential to reduce patients' IgE binding to the major birch pollen allergen, Bet v 1

Ines Zettl, Tatiana Ivanova, Maria R. Strobl, Christina Weichwald, Oksana Goryainova, Evgenia Khan, Marina V. Rutovskaya, Margarete Focke- Tejkl, Anja Drescher, Barbara Bohle, Sabine Flicker, Sergei V. Tillib Allergy 2022...[more]

 

Impaired Mineral Ion Metabolism in a Mouse Model of Targeted Calcium-Sensing Receptor (CaSR) Deletion from Vascular Smooth Muscle Cells

Martin Schepelmann (group Enikö Kallay) and national and international colleagues and collaborators have just published a study in the Journal of the American Society of Nephrology (JASN), one of the highest ranked and most...[more]

 

Vaccine based on folded RBD-PreS fusion protein with potential to induce sterilizing immunity to SARS-CoV-2 variants

The preclinical data for a vaccine developed at MedUni Vienna to protect against SARS-CoV-2 indicates that it is effective against all SARS-CoV-2 variants known to date, including omicron - even in those who have not yet built up...[more]

 

Birch pollen allergic patients have IgE and IgG antibodies binding to diverse patterns of conformational epitopes on the major allergen, Bet v 1

Schmalz S, Mayr V, Shosherova A, Gepp B, Ackerbauer D, Sturm G, Bohle B, Breiteneder H, Radauer C. Isotype-specific binding patterns of serum antibodies to multiple conformational epitopes of Bet v 1.[more]

 

Neutralization of SARS-CoV-2 requires antibodies against conformational receptor-binding domain epitopes.

Gattinger P, Niespodziana K, Stiasny K, Sahanic S, Tulaeva I, Borochova K, Dorofeeva Y, Schlederer T, Sonnweber T, Hofer G, Kiss R, Kratzer B, Trapin D, Tauber PA, Rottal A, Körmöczi U, Feichter M, Weber M, Focke-Tejkl M,...[more]

 
Displaying results 1 to 7 out of 98
<< First < Previous 1 2 3 4 5 6 7 Next > Last >>
 
Inhaltsbereich

Fusion proteins consisting of Bet v 1 and Phl p 5 form IgE-reactive aggregates with reduced allergenic activity

N. Najafi, G. Hofer, P. Gattinger, D. Smiljkovic, K. Blatt, A. Stöcklinger, W. Keller, P. Valent, J. Thalhamer, R. Valenta, S. Flicker., Scientific Reports, 9(1):4006. doi: 10.1038/s41598-019-39798-8.

Immunoglobulin E (IgE)-mediated allergy is the most common immunological hypersensitivity disease. The prevalence has been continuously rising over the last decades and currently, more than 25% of the population is affected. IgE-allergen complexes induce mast cell and basophil activation and thus immediate allergic inflammation.

Major birch and grass pollen allergens, Bet v 1 and Phl p 5 represent two of the most common allergens that induce strong allergic reactions in allergic patients as already repeatedly demonstrated by skin and nasal provocation testing.

To understand the potency of allergens to induce such severe allergic symptoms Dr. Nazanin Najafi from the group of Assoc. Prof. Sabine Flicker started to investigate different properties of the highly potent allergens, Phl p 5 and Bet v 1. In her study, which was recently published in Scientific Reports, a hybrid protein consisting of the complete mature sequence of Phl p 5 fused to the complete sequence of Bet v 1 was constructed, expressed and purified. Since each of these allergens occurs as soluble and monomeric protein, they expected the hybrid protein to remain fully IgE-reactive, allergenic and monomeric. Size exclusion experiments revealed that the majority of hybrid protein appears as high molecular weight aggregates of more than 1000 KDa. These results were in correspondence with illustrations obtained from negative stain electron microscopy. Hybrid, as an aggregated protein compared with equimolar mix of Phl p 5 and Bet v 1, exhibited a stronger IgE reactivity with sera from patients who were allergic to birch and grass pollen. However, to their surprise, basophil activation tests with above mentioned patients, demonstrated a reduced allergenic activity of hybrid compared with that of equimolar mix of the isolated allergens.

This study demonstrates clearly that a considerable reduction of allergenic activity can also be achieved through molecular aggregation of allergens not only by various recombinant DNA technologies such as mutation, reassembly, or fragmentation. Dr. Najafi explains the reduction of allergenic activity of the hybrid through a reorientation of the spatial arrangement of IgE epitopes in an unfavorable position for cross-linking of effector cell-bound IgE antibodies. Therefore the hybrid protein could be of clinical impact as controlled molecular aggregation may be used as a new technology for reducing the allergenic activity of allergens and thus for the engineering of a new type of allergy vaccines.

Link zu Pubmed

 
 
Drucken
 

Schnellinfo

 
-- Green IPA
-- Technology Platforms
-- Kontakt
-- News (Archiv)
-- Seminare
-- Social Life
-- Intranet Login
-- QM-Dokumente (Intranet)
-- Bildergalerie (Intranet)
-- Alumni
-- Links
 
 

Featured

 
 
 
© MedUni Wien  | 
 Impressum | Nutzungsbedingungen | Datenschutzerklärung | Barrierefreiheit | Kontakt