Anaesthesia,General Intensive Care and Pain Management
Current academic degree
Dr. med. univ.
Medical University of Innsbruck
Cyclic Changes in PO2 as a Mechanism of Lung Injury
Lung injury during mechanical ventilation is a serious issue in intensive care medicine. Ventilator-associated lung injury (VALI) can be the result of mechanical stress caused by recruitment and derecruitment of atelectasis (R/D) of the lungs. This repetitive R/D during the respiratory cycle leads to intermittent changes of oxygen partial pressure (pO2) resulting in oxidative stress to local tissue and systemic inflammation.
The aims of this project are to study the effects of cyclic changes of oxygen partial pressure on mice and human lung cell cultures. Therefore, we will employ and refine an in vitro model developed in this research group (Medical University Vienna), which can provide fast alternated gas exposure via gas permeable membranes. The hypothesis of this project is defined as: “Rapid changes of pO2 in the lungs represent an independent mechanism of lung-biotrauma and injury”.
Cell cultures of mice pulmonary endothelial cells and human alveolar type 1 and type 2 cells will be exposed to different oxygen concentrations, in addition to oscillating oxygen levels.
The harmful effects on cell cultures will be assessed by quantification of apoptotic and necrotic states, oxidative stress, inflammation and activation of key transcript factors and downstream pathways. In addition, potential beneficial effects using antioxidant and anti-inflammatory drugs will be tested.
This project is a FWF-stand-alone project (P28618-B28, Gottfried and Vera Weiss Prize)