The Laccone Lab is using methods such as Dynamic Light Scattering (DLS), Small Angle X-Ray Scattering (SAXS) and Nuclear Magnetic Resonance (NMR) spectroscopy to investigate protein stability (both conformational and otherwise), aggregation propensity and the presence of folded domains. Stable, non-aggregating protein constructs at optimal buffer conditions yield more favorable results in downstream assays, such as cell culture experiments
The application of a recombinant protein for a possible treatment of RETT syndrome has been patented: “Synthetic MECP2 sequence for protein substitution therapy“, (WO/2007/115578). However, we also attempt to develop and investigate recombinant proteins as a possible strategy for other genetic diseases.
Furthermore, Franco Laccone is interested in recruitment of patients with unknown illnesses for their identification.