Professor of Atherosclerosis Research
Department of Laboratory Medicine
Medical University of Vienna
Center for Molecular Medicine of the Austrian Academy of Sciences
Lazarettgasse 14, AKH BT25.2, A-1090 Vienna
Phone: +43 (0)1 40400 - 73755
FAX: +43 (0)1 40400 - 73588
*Immune mechanisms of atherosclerosis *Lipid-peroxidation derived structures as targets of innate immunity *Natural antibodies in health and disease.
Immunity and Atherosclerosis
Dr. Binder is interested in the role of immune mechanisms in atherosclerosis and the immune recognition of oxidation-specific epitopes. His interests are clearly interdisciplinary and span vascular biology, lipid oxidation, natural antibodies and innate immunity. In the past years, Dr. Binder’s research has focused on the role of natural IgM antibodies in atherosclerosis. He first described the atheroprotective role of the natural IgM antibody T15/EO6, which was followed by studies defining protective mechanisms of T15/EO6. This included the demonstration that a large part of natural IgM in mice and humans is directed against oxidation-specific epitopes, and the characterization of germline encoded natural IgM antibodies that are derived from innate B-1 cells. Currently, his laboratory is defining the functional role of natural IgM antibodies using mouse models of atherosclerosis and is working to identify molecular mechanisms that lead to the production of these antibodies by B-1 cells.
A particular emphasis is put on identifying ways how these responses can be exploited for atheroprotective interventions. Major efforts focus on the recent discovery that a large part of natural IgM and complement factor H bind malondialdehyde-epitopes and protect against the consequences of oxidative stress. In addition, Dr. Binder’s research interests include the role of circulating microparticles as carriers of biologically active oxidized lipids and as targets of innate immunity.
The group utilizes mouse models of atherosclerosis in combination with various immune deficient models for morphometric analyses of the extent of atherosclerosis and assessment of lesion phenotype by immunohistochemistry. Major techniques used include a wide array of immunological assays, flow cytometry, FACSorting, adoptive in vivo cell transfer and bone marrow transplantation; the biochemical generation of oxidized lipid antigens/ligands and standard molecular biology and cell culture techniques. Group members have access to a fully equipped cell culture facility, FACS sorter and analyzers, microtiter plate readers, ultracentrifuge, HPLC, FPLC, PCR equipment (incl. multichannel Real-time PCR), next generation sequencing equipment, cryo- and microtome, and a fluorescent microscope with imaging equipment.
More information about the various research projects can be found <here>
Amir S, Hartvigsen K, Gonen A, Leibundgut G, Que X, Jensen-Jarolim E, Wagner O, Tsimikas S, Witztum JL, Binder CJ. Peptide mimotopes of malondialdehyde-epitopes for clinical applications in cardiovascular disease. J Lipid Res 2012; 53(7):1316-26
Cardilo-Reis L, Gruber S, Schreier SM, Drechsler M, Papac-Milicevic N, Weber C, Wagner O, Stangl H, Soehnlein O, Binder CJ. Interleukin-13 protects from atherosclerosis and modulates plaque composition by skewing the macrophage phenotype. EMBO Mol Med 2012; 4(10):1072-86.
Weismann, D., K. Hartvigsen, N. Lauer, K. L. Bennett, H. P. Scholl, P. Charbel Issa, M. Cano, H. Brandstatter, S. Tsimikas, C. Skerka, G. Superti-Furga, J. T. Handa, P. F. Zipfel, J. L. Witztum, and C. J. Binder. Complement factor H binds malondialdehyde epitopes and protects from oxidative stress. Nature 2011; 478: 76-81.