We are interested in elucidating the immunological mechanisms underlying the induction, maintenance and effective treatment of IgE-mediated allergy. Moreover, we characterize allergens and develop novel concepts for allergen-specific immunotherapy.
Techniques, methods & infrastructure
Our expertise includes a broad spectrum of state-of-the-art-methodologies in cellular and molecular biology and immunology, e.g. human cell culture, magnetic cell sorting, FACS, intracellular cytokine detection, real-time PCR, live cell imaging, T cell cloning and T cell epitope mapping systems, ELISA, and immunoblotting.
We are specialized in the isolation and characterization of primary immune cells (T, B, DC, Monocytes), and allergen-specific T cell lines and clones. In addition, we perform various read-out systems for allergic responses, e.g. basophil activation assays, mediator-release experiments, facilitated allergen-binding assays, and study antigen-processing by means of antigen-uptake and degradation assays, endolysosomal proteolysis, and presentation to specific T cells.