Bioactive peptides from natural sources: Distribution, evolution and isolation of circular plant peptides (cyclotides); Peptidomics of plant cyclotides; Pharmacology of plant cyclotides; G-protein coupled receptors as targets for disulfide-rich peptides from plants and marine cone-snails.
Novel immunosuppressant circular peptides from plants: Bio-/Chemical analysis of immunosuppressive properties of cyclotides; Peptidomics of circular plant peptides; anti-proliferative effect on T-lymphocytes; therapeutic potential in vitro and in vivo.
GPCR interacting proteins, folding and signaling: Tandem-affinity purification and proteomic interactome mapping of adenosine receptors and related GPCRs; folding and pharmacochaperoning of adenosine receptors.
Molecular signaling mechanisms of bioactive peptides from nature
Cell culture and receptor pharmacology (ligand binding, function activation); affinity purification, Western blotting; peptide extraction and isolation, HPLC, mass spectrometry (nano-LC-MS/MS, MALDI-TOF/TOF) and proteomics;
5 selected publications
Gruber and Muttenthaler (2012) Public Library of Science ONE, 7: e32559. Gründemann et al. (2012) Journal of Natural Products, 75: 167-74. Gruber et al. (2010) Current Pharmaceutical Design, 16: 3071-88. Gruber et al. (2008) Plant Cell, 20: 2471-83. Gruber et al. (2007) Journal of Biological Chemistry, 282: 20435-46.