Dr. Kubista’s research interest focuses on the role of various ion channels in the electrical activities of nerve cells. The general aim of his work is to understand the implication of selected ion channels in normal and abnormal discharge activities and to assess potential targetability of these channels in neurological diseases. Currently Dr. Kubista’s group is investigating these questions with respect to L-type voltage-gated calcium channels. Using in vitro-models of epileptiform activity („epilepsy in the culture dish“) the group address whether these calcium channels can be used as targets for anti-epileptic or anti-epileptogenic therapy.
Dr. Kubista’s group uses primarily current-clamp experiments on primary rat and mouse central neurons to record membrane voltage in the minimally invasive perforated-patch mode. In addition, voltage clamp experiments are employed for further analysis. All facilities for the above mentioned electrophysiological measurements and for preparation and maintenance of primary neuronal cultures are available in the lab.
Geier P, Lagler M, Boehm S, Kubista H. Dynamic interplay of excitatory and inhibitory coupling modes of neuronal L-type calcium channels. Am J Physiol Cell Physiol. 2011 Apr;300(4):C937-49. PubMed PMID: 21228322.
Kubista H, Kosenburger K, Mahlknecht P, Drobny H, Boehm S. Inhibition of transmitter release from rat sympathetic neurons via presynaptic M(1) muscarinic acetylcholine receptors. Br J Pharmacol. 2009 Apr;156(8):1342-52. PubMed PMID: 19309359.
Kubista H, Mafra RA, Chong Y, Nicholson GM, Beirão PS, Cruz JS, Boehm S, Nentwig W, Kuhn-Nentwig L. CSTX-1, a toxin from the venom of the hunting spider Cupiennius salei, is a selective blocker of L-type calcium channels in mammalian neurons. Neuropharmacology. 2007 Jun;52(8):1650-62. PubMed PMID: 17517422.
Kubista H, Edelbauer H, Boehm S. Evidence for structural and functional diversity among SDS-resistant SNARE complexes in neuroendocrine cells. J Cell Sci. 2004 Feb 29;117(Pt 6):955-66. PubMed PMID: 14762114.
Kubista H, Lechner SG, Wolf AM, Boehm S. Attenuation of the P2Y receptor-mediated control of neuronal Ca2+ channels in PC12 cells by antithrombotic drugs. Br J Pharmacol. 2003 Jan;138(2):343-50. PubMed PMID: 12540525.