I am interested in the structure and function of surface receptors on T cells and accessory/dendritic cells to identify novel targets for influencing abnormal and unwanted immune reactions in immunological disorders and diseases. Internationally well recognized are the investigations, which contribute to the understanding of how glycosylphosphatidylinositol (GPI)-anchored receptor proteins transduce signals across the plasma membrane. These studies were fundamental for the identification and characterization of special membrane microdomains, called lipid rafts, which are more and more characterized to control initiation of signal transduction across the plasma membrane of cells. Currently, I put special emphasis on the signal transduction mechanism of the T cell antigen receptor (TCR), in particular how Lck, the key signalling kinase, is regulated as well as how positive and negative regulating transmembrane accessory molecules control TCR signalling.
techniques and infrastructure of the research group:
Cell culture, multi-parameter flow cytometry, molecular cloning, shRNA silencing, immunological techniques (isolation, cultivation and stimulation of immune cells, ELISA, immunofluorescence, Western blotting, immunoprecipitation, etc), chromatography, advanced imaging (single molecule analysis, confocal microscopy)
1. Schwarzenbacher, M., M. Kaltenbrunner, M. Brameshuber, C. Hesch, W. Paster, J. Weghuber, B. Heise, A. Sonnleitner, H. Stockinger*, and G. J. Schütz*. 2008. Micropatterning for quantitative analysis of protein-protein interactions in living cells. Nature Meth. 5:1053-1060 *Corresponding author.
2. Paster, W., C. Paar, P. Eckerstorfer, A. Jakober, K. Drbal, G. J. Schütz, A. Sonnleitner, and H. Stockinger. 2009. Genetically encoded FRET-sensors for the conformation of the Src-family kinase Lck. J. Immunol. 182:2160–2167.
3. Brameshuber, M., J. Weghuber, V. Ruprecht, I. Gombos, I. Horváth, L. Vigh, P. Eckerstorfer, E. Kiss, H. Stockinger, and G. J. Schütz. 2010. Imaging of mobile long-lived nanoplatforms in the live cell plasma membrane. J. Biol. Chem. 285:41765-41771.
4. Leksa, V., R. Loewe, B. Binder, H. B. Schiller, P. Eckerstorfer, F. Forster, A. S. Cardona, G. Ondrovičová, E. Kutejová, B. R. Binder, and H. Stockinger. 2011. Soluble M6P/IGF2R released by TACE controls angiogenesis via blocking plasminogen activation. Circulation Res. 108:676-685.
5. Schatzlmaier, P., V. Supper, L. Göschl, A. Zwirzitz, P. Eckerstorfer, E. Ellmeier, J. B. Huppa, and H. Stockinger. 2015. Rapid multiplex analysis of lipid raft components with single cell resolution. Science Signaling. In press.