Dr. Böhm is interested in the molecular mechanisms and functional consequences of the modulation of transmitter release via presynaptic receptors. He has pursued this research topic by investigating central and peripheral neurons. In the recent past, the focus of research was the identification of autoregulatory nucleotide receptors that mediate the feedback control of transmitter release from sympathetic neurons. In hippocampal neurons, Dr. Böhm investigated inhibitory presynaptic auto- and heteroreceptors and is now interested in presynaptic transmitter-gated as well as voltage-gated ion channels. In addition to presynaptic mechanisms, the molecular pharmacology of neuronal ion channels and P2Y receptors is a research interest of Dr. Böhm.
The group uses primary cultures of mouse and rat hippocampal, spinal cord, sensory, and sympathetic neurons (Böhm and Betz, 1997; Böhm 1999) for transfection (Goetze et al, 2005), for the recording of autaptic and miniature synaptic currents (Böhm and Betz, 1997), of currents through Ca2+ and Kv7 channels (Lechner et al, 2003 and 2005), and for the determination of transmitter release via radiotracers (Böhm, 1999). Intracellular second messengers (cAMP and inositol phosphates) are quantified by radioactive labelling and subsequent separation by column chromatography (Moskvina et al, 2003). FRET of fusion proteins containing CFP and YFP is done via fluorescent microscopy (Schicker et al, 2008).
Members of this research group have access to two complete cell culture labs (preparative microscope, sterile hoods, two incubators, fluorescence microscopes), one standard radioactivity lab, one standard biochemistry and molecular biology lab, a fluorescence microscope with monochromator, filter wheels, and CCD camera, one confocal laser scanning microscope, and 5 complete patch clamp rigs.
Böhm S (1999) ATP stimulates sympathetic transmitter release via presynaptic P2X purinoceptors. J Neurosci 19:737-746.
O'Connor V, El Far O, Bofill-Cardona E, Nanoff C, Freissmuth M, Karschin A, Airas JM, Betz H, Böhm S (1999) Calmodulin dependence of presynaptic metabotropic glutamate receptor signaling. Science 286:1180-1184.
Kubista H, Edelbauer H, Böhm S (2004) Evidence for structural and functional diversity among SDS-resistant SNARE complexes in neuroendocrine cells. J Cell Sci 117:955-966.
Schicker KW, Chandaka GK, Geier P, Kubista H, Böhm S (2010) P2Y1 receptors mediate an activation of neuronal calcium-dependent K+ channels. J Physiol 588: 3713-3725.
Yousuf A, Klinger F, Schicker KW, Böhm S (2011) Nucleotides control the excitability of sensory neurons via two P2Y receptors and a bifurcated signalling cascade. Pain 152: 1899-1908.