Assoc.Prof. MMag. Dr. rer. nat. Alice Assinger
Institute of Physiology
Centre of Physiology and Pharmacology
Schwarzspanierstrasse 17, A-1090 Vienna, Austria
Phone: +43 1 40160 31405
know-how and research interests:
We are interested in the physiological and pathophysiological roles of platelets in inflammation, infection and tissue regeneration. Platelets are circulating anucleate blood cells that have a critical function in haemostasis by facilitating the cessation of bleeding. In recent years it became increasingly apparent that platelets are not only important in haemostasis and thrombosis, but they can also fine-tune immune responses as well as tissue repair processes. Upon activation platelets release the content of their granules, which contain a plethora of downstream effector molecules that can modulate leukocyte and endothelial cell functions. These include cytokines and chemokines, coagulation factors and fibrinolytic agents, as well as regulators of angiogenesis.
Our group investigates the immunomodulatory role of platelets in acute and chronic inflammation and infection, the mechanism of platelet-mediated tissue regeneration and the impact of antiplatelet therapies in this context. Moreover we focus on novel biomarker development to predict disease progression in cardiovascular and liver diseases, cancer and sepsis.
Platelets and microvesicles in infection, inflammation and tissue regeneration
techniques and infrastructure of the research group:
- Characterisation of various leukocyte subsets and activation status (mouse and human) via flow cytometry and fluorescence microscopy
- Live cell microscopy under flow conditions for recruitment and extravasation assays
- Microvesicle characterisation
- Isolation, characterization and cultivation of human and murine primary cells (platelets, leukocyte subsets, various endothelial cells and hepatocytes)
- Biomarker research (collection and analysis of biorepositories) for microvesicle, microRNA and metabolite analysis
- Thrombosis and haemostasis animal models (FeCl3 and mechanic injury, tail bleeding)
- Inflammation and regeneration animal models (viral infections, lung injury, peritonitis, microRNA and antagomir treatment, partial and extended hepatectomies)
- Transgenic mice for various platelet function deficiencies
- Platelet function analysis (e.g. light transmission aggregometry)
- Standard biomolecular tools (PCR, qPCR, ELISA, Western Blot, cell culture)
5 selected publications:
1. Kral-Pointner, J B; Schrottmaier, W C; Salzmann, M; Mussbacher, M; Schmidt, G; Moser, B A; Heber, S; Birnecker, B; Paar, H; Zellner M.; Knapp, S; Assinger, A; Schabbauer, G; Platelet PI3K modulates innate leukocyte extravasation during acid-induced acute lung inflammation. Thrombosis and Haemostasis. 2019. doi 10.1160/TH19-01-0031
2. Starlinger P, Hackl H, Pereyra D, Skalicky S, Geiger E, Finsterbusch M, Tamandl D, Brostjan C, Grunberger T, Hackl M, Assinger A. Predicting Postoperative Liver Dysfunction Based on Blood-Derived MicroRNA Signatures. Hepatology (Baltimore, Md). 2019. Epub 2019/02/20. doi: 10.1002/hep.30572.
3. Starlinger P, Haegele S, Offensperger F, Oehlberger L, Pereyra D, Kral JB, Schrottmaier WC, Badrnya S, Reiberger T, Ferlitsch A, Stift J, Luf F, Brostjan C, Gruenberger T, Assinger A. The profile of platelet alpha-granule released molecules affects postoperative liver regeneration. Hepatology (Baltimore, Md). 2016;63(5):1675-88. Epub 2015/11/04. doi: 10.1002/hep.28331.
4. Schrottmaier, W. C., Kral, J. B., Badrnya, S., & Assinger, A. (2015). Aspirin and P2Y12 Inhibitors in platelet-mediated activation of neutrophils and monocytes. Thromb Haemost, 114(3), 478-489. doi:10.1160/TH14-11-0943
5. Badrnya, S., Schrottmaier, W. C., Kral, J. B., Yaiw, K. C., Volf, I., Schabbauer, G., . . . Assinger, A. (2014). Platelets mediate oxidized low-density lipoprotein-induced monocyte extravasation and foam cell formation. Arterioscler Thromb Vasc Biol, 34(3), 571-580. doi:10.1161/ATVBAHA.113.302919