Ao. Prof. Dr. Johannes Breuss
Department of Vascular Biology and Thrombosis Research
Center of Physiology and Pharmacology
Schwarzspanierstrasse 17, 1090 Vienna, Austria
Phone: +43 (0)1 40160 - 31115
Know-how and research interests
My group is interested in the pro-angiogenic properties of the urokinase receptor and in plant derived anti-inflammatory substances.
The role of the urokinase receptor in tumour angiogenesis
The urokinase receptor is responsible for the activation of peri-cellular proteolysis enabling cells to invade tissue by proteolytic digestion of extracellular matrix and to migrate on a variety of extracellular matrix proteins. Endothelial cells utilize this receptor for the formation of endothelial sprouts and angiogenesis. We investigate the contribution of the urokinase receptor to tumour angiogenesis by an additional mechanism – the redistribution of integrins, receptors enabling cell migrtion and survival. For this we use transgenic mouse models that facilitate the assessment of tumor angiogenesis.
Anti-inflammatory properties of plant derived substances.
The second focus of our research is directed to the elucidation of anti-inflammatory properties of plant derived substances. We investigate constituents of the oil from the seeds of Nigella sativa and – in collaboration with groups participating in the national research network project DNTI (Drugs from Nature Targeting Inflammation) we investigate extracts from a spectrum of plants for their inhibitory effect on inflammation related pathways such as the NFkappaB pathway. Focusing on vascular inflammation we utilize primary endothelial cells and murine models of inflammation such as the femoral artery cuff model to establish the activity of lead substances provided by the DNTI consortium.
Techniques and infrastructure of the research group
Cell culture of primary human and murine endothelial cells and tumor cell lines, transgenic animals, immunohisto- and cytochemistry, flow cytometry, confocal microscopy, video-based cell migration and invasion, peptide based assays, Western blotting, ELISA, pathway analysis, real time PCR, in situ hybridization
5 selected publications:
Breuss,J.M. and Uhrin,P. VEGF-initiated angiogenesis and the uPA/uPAR system. Cell Adh. Migr. 6., [Epub ahead of print] 2012.
Papac-Milicevic,N., Breuss,J.M., Zaujec,J., Ryban,L., Plyushch,T., Wagner,G.A., Fenzl,S., Dremsek,P., Cabaravdic,M., Steiner,M., Glass,C.K., Binder,C.J., Uhrin,P., and Binder,B.R. The interferon stimulated gene 12 inactivates vasculoprotective functions of NR4A nuclear receptors. Circ. Res. 110, e50-e63, 2012.
Alexander,R.A., Prager,G.W., Mihaly-Bison,J., Uhrin,P., Sunzenauer,S., Binder,B.R., Schutz,G.J., Freissmuth,M., and Breuss,J.M. VEGF-induced endothelial cell migration requires urokinase receptor (uPAR)-dependent integrin redistribution. Cardiovasc. Res. 94, 125-135, 2012.
Geetha,N., Mihaly,J., Stockenhuber,A., Blasi,F., Uhrin,P., Binder,B.R., Freissmuth,M., and Breuss,J.M. Signal integration and coincidence detection in the mitogen-activated protein kinase/extracellular signal-regulated kinase (ERK) cascade: concomitant activation of receptor tyrosine kinases and of LRP-1 leads to sustained ERK phosphorylation via down-regulation of dual specificity phosphatases (DUSP1 and -6). J. Biol. Chem. 286, 25663-25674, 2011.
Breuss,J.M., Cejna,M., Bergmeister,H., Kadl,A., Baumgartl,G., Steurer,S., Xu,Z., Koshelnick,Y., Lipp,J., de,M.R., Losert,U., Lammer,J., and Binder,B.R.. Activation of nuclear factor-kappa B significantly contributes to lumen loss in a rabbit iliac artery balloon angioplasty model. Circulation 105, 633-638. 2002