Univ. Prof. DI Dr. Viktoria Weber
Department of Health Sciences and Biomedicine
Danube University Krems
Dr.-Karl-Dorrekstr. 30, A-3500 Krems, Austria
Phone: +43 2732 893 2632
know-how and research interests:
My group is interested in the development of supportive therapies for sepsis. Within the Christian Doppler Laboratory for Innovative Therapy Approaches in Sepsis, we have set up various cell culture models to monitor lipopolysaccharide-induced endothelial activation. We study the role of extracellular vesicles (exosomes, microvesicles) as markers for cellular activation and have established a range of methods to isolate extracellular vesicles and to characterize them with respect to their cellular origin and their biological activity (immunomodulation, activation of coagulation). We also deal with the role of microvesicles as indicators of blood compatibility during blood material interaction, e.g. during contact of blood with adsorbents in apheresis.
research topic (general title):
Endothelial activation in sepsis, role of extracellular vesicles in inflammation and as markers of cellular activation during blood-material interaction.
techniques and infrastructure of the research group:
Diverse biochemical and cellular biology techniques, immunological and functional assays, flow cytometry, isolation and characterziation of extracellular vesicles (exososomes and microvesicles), assays for the biological activity of exososmes and microvesicles (immunomodulation and coagulation), nanoparticle tracking analysis, cell culture models for endothelial activation, electrophoresis and western blotting, thrombin generation assays, apheresis.
5 selected publications:
Weiss R, Spittler A, Schmitz G, Fischer MB, Weber V (2014) Thrombocyte adhesion and release of extracellular microvesicles correlate with surface roughness of adsorbent polymers for lipid apheresis.
Schildberger A, Rossmanith E, Eichhorn T, Strassl K, Weber V (2013) Monocytes, Peripheral blood mononuclear cells, and THP-1 cells exhibit different cytokine expression patterns following stimulation with lipopolysaccharide.
Mediators of Inflammation 2013:697972
Tripisciano C, Leistner A, Linsberger I, Leistner A, Falkenhagen D, Weber V (2012) Effect of anticoagulation with citrate versus heparin on the adsorption of coagulation factors to blood purification resins with different charge.
Eifler R, Lind J, Falkenhagen D, Weber V, Fischer MB, Zeillinger R (2011) Enrichment of circulating tumor cells from a large blood volume using leukapheresis and elutriation: a proof of concept.
Clinical Cytometry 80(2):100-111
Cantaluppi V, Weber V, Lauritano C, Figliolini F, Beltramo S, Biancone L, Del Cal M, Cruz D, Ronco C, Segolini GP, Tetta C, Camussi G (2010) Protective effect of resin adsorption on septic plasma-induced tubular injury.
Critical Care 14(1):R4