Mag. Dr. Wolfgang Holnthoner
Ludwig-Boltzmann-Institute for Experimental and
Clinical Traumatology, Austrian Cluster for Tissue
Donaueschingenstrasse 13, A-1200 Vienna, Austria
Phone: +43 5 93 93-41960
know-how and research interests:
→Being trained as endothelial cell biologist, I am interested in the basic functions of these cells in vasculogenesis, angiogenesis and lymphangiogenesis. Moreover, in a translational setting my team and I are working on vascularization strategies to engineer artificial vascular structures and to regenerate existing blood and lymphatic vessels. Furthermore, the group spans many aspects of endothelial cell biology:
- Isolation of endothelial progenitor cells (EPC) from peripheral blood and fat tissue
- Co-cultures as tools for prevascularization for Tissue Engineering
- De- and recelluarization of natural vascular structures
- The role of endothelial cells in blood coagulation
→The role of endothelial cells in tissue engineering and regenerative medicine
techniques and infrastructure of the research group:
→ Diverse basic molecular and cellular biology techniques, in vitro and in vivo angiogenesis and vasculogenesis assays, flow cytometry, immunohistochemistry, confocal microscopy, co-culture systems for vascularization in fibrin matrices, decellularization techniques for natural vascular structures, thromoboelastometry.
5 selected publications:
Molecular and cellular effects of in vitro shockwave treatment on lymphatic endothelial cells.
Rohringer S, Holnthoner W, Hackl M, Weihs AM, Rünzler D, Skalicky S, Karbiener M, Scheideler M, Pröll J, Gabriel C, Schweighofer B, Gröger M, Spittler A, Grillari J, Redl H.
PLoS One. 2014 Dec 11;9(12):e114806
Mechanisms of vasculogenesis in 3D fibrin matrices mediated by the interaction of adipose-derived stem cells and endothelial cells. Rohringer S, Hofbauer P, Schneider KH, Husa AM, Feichtinger G, Peterbauer-Scherb A, Redl H, Holnthoner W. Angiogenesis. 2014 Oct;17(4):921-33
Tvorogov D, Anisimov A, Zheng W, Leppänen VM, Tammela T, Laurinavicius S, Holnthoner W, Heloterä H, Holopainen T, Jeltsch M, Kalkkinen N, Lankinen H, Ojala PM, Alitalo K. Effective Suppression of Vascular Network Formation by Combination of Antibodies Blocking VEGFR Ligand Binding and Receptor Dimerization. Cancer Cell. 2010 Dec 14; 18: 630-40
Petrova TV, Bono P, Holnthoner W, Sihto H, P Laakkonen P, Heikkilä P, Joensuu H, Alitalo K. VEGFR-3 expression is restricted to blood and lymphatic vessels in solid tumors. Cancer Cell. 2008 Jun; 13(6):554-6
Holnthoner W, Pillinger M, Groger M, Wolff K, Ashton AW, Albanese C, Neumeister P, Pestell RG, Petzelbauer P. Fibroblast growth factor-2 induces Lef/Tcf-dependent transcription in human endothelial cells. J Biol Chem 2002 Nov 29; 277(48):45847-53