Priv. Doz. Mag. Dr. Wolfgang Holnthoner
Ludwig-Boltzmann-Institute for Experimental and
Clinical Traumatology, Austrian Cluster for Tissue
Donaueschingenstrasse 13, A-1200 Vienna, Austria
Phone: +43 5 93 93-41960
know-how and research interests:
Being trained as endothelial cell biologist, I am interested in the basic functions of these cells in vasculogenesis, angiogenesis and lymphangiogenesis. My group's primary focus is the regeneration/engineering of microvascular structures. To this aim we employ co-culturing primary endothelial cells with supporting cell types to achieve functional oxygen and nutrient support of tissues. We use the physiological and biocompatible scaffold fibrin to provide the cells the necessary 3D-environment for microvascularization. In addition, our research involves the integration of lymphatic vessels, since they are the ones, which transport the interstitial fluid in tissues back to the blood circulation, and without them human life would be inconceivable. Moreover, we strive to explore the emerging role of extracellular vesicles (EV) in intercellular communication. These efforts contribute to the development of therapies aimed for regeneration of diseased tissues.
The role of blood and lymphatic endothelial cells in tissue engineering and regenerative medicine
techniques and infrastructure of the research group:
Diverse basic molecular and cellular biology techniques, in vitro and in vivo angiogenesis and vasculogenesis assays, flow cytometry, immunohistochemistry, confocal microscopy, co-culture systems for vascularization in fibrin matrices, thromoboelastometry, nanoparticle tracking analysis (NTA)
5 selected publications:
Mühleder S, Fuchs C, Basilio J, Szwarz D, Pill K, Labuda K, Slezak P, Siehs C, Proell J, Priglinger E, Hoffmann C, Junger W, Redl H, Holnthoner W. Purinergic P2Y2 receptors modulate endothelial sprouting. Cell Mol Life Sciences 2019, doi.org/10.1007/s00018-019-03213-2
Schneider J, Pultar M, Holnthoner W. Ex vivo engineering of blood and lymphatic vascular networks. Vascular Biology 2019. 1:1, H17-H22.
Holnthoner W, Bonstingl C, Hromada C, Muehleder S, Zipperle J, Stojkovic S, Redl H, Wojta J, Schoechl H, Grillari J, Weilner S, Schlimp C. Endothelial Cell-derived Extracellular Vesicles Size-dependently Exert Procoagulant Activity Detected by Thromboelastometry. Sci Rep. 2017 ;7:
Knezevic L, Schaupper M, Mühleder S, Schimek K, Hasenberg T, Marx U, Priglinger E, Redl H, Holnthoner W. Engineering Blood and Lymphatic Microvascular Networks in Fibrin Matrices. Front. Bioeng. Biotechnol. 2017 Apr 18;5:25
Rohringer S, Hofbauer P, Schneider K, Husa AM, Feichtinger G, Peterbauer-Scherb A, Redl H, Holnthoner W. Molecular mechanisms of vasculogenesis in 3D fibrin matrices mediated by the interaction of adipose-derived stem cells and endothelial cells. Angiogenesis. 2014 Oct;17(4):921-33