Supervisor: Reinhard Gruber
Committee: Ulrike Kuchler, Stefan Krautwald
Department: Oral Biology
E-mail: layla.panahipour@meduniwien.ac.at
Tel: +43 (0)1 40070 - 5202
Current academic degree: D.D.S., Dr.scient.med.
Previous University and Subject: Medical University of Vienna
Thesis since: 03/2021
Periodontitis is an inflammatory process being associated with necroptosis, a molecular process that requires caspase-8 for activation of receptor-interacting serine/threonine-protein kinase 1 (RIPK1), RIPK3 and mixed-lineage kinase domain-like protein (MLKL). MLKL mediates one programmed cell death process, the necroptosis pathway. In periodontitis, necroptosis is supposed to influence bacterial infection progression and presumably plays a role in maintaining the homeostasis in the periodontal niche. In gingival tissues collected from patients with untreated chronic periodontitis, elevated levels of MLKL and phosphorylated MLKL were observed. Moreover, periodontitis models showed increased phosphorylated MLKL in biopsies while pharmacological blocking MLKL reduced inflammatory osteolysis. There is thus accumulating evidence that necroptosis is among the pathologic mechanisms of periodontitis. This evidence, however, remains descriptive and the role of MLKL in the progression and regeneration of the periodontal tissue is unknown. Thus, no information is available if MLKL signaling is required for the healing of extraction sockets and the process of periodontal inflammatory osteolysis. Thus, it is relevant to investigate the role of MLKL in alveolar bone regeneration. Moreover, there is a demand to further study the involvement of MLKL in periodontal destruction upon inflammation. Based on this missing knowledge we have identified three research objectives:
cell culture; PCR; ELISA; immunostaining; Western blot; micro-CT
Panahipour L, Omerbasic A, Nasirzade J, Gruber R: TGF-β activity of a demineralized bone matrix.Int J Mol Sci 22: 664, 2021
Panahipour L, Kargarpour Z, Luza B, Lee J-S, Gruber R: TGF-β activity related to the use of collagen membranes: In vitro bioassays. Int J Mol Sci 21: 6636, 2020
Panahipour L, Tabatabaei AA, Gruber R: Hypoallergenic infant formula lacks transforming growth factor beta activity and has a lower anti-inflammatory activity than regular infant formula. J Dairy Sci 103: 6771-6781, 2020
Panahipour L, De Biasi M, Bokor TS, Thajer A, Haiden N, Gruber R: Milk lactoperoxidase decreases ID1 and ID3 expression in human oral squamous cell carcinoma cell lines. Sci Rep 10: 5836, 2020