PATEISKY Petra

Molecular/Cellular Abberations in the Course and Development of Endometriosis

Endometriosis is an estrogen-driven, multifactorial disease affecting women in their reproductive age. Changes in immunologic, angiogenic, inflammatory and genetic as well as epigenetic factors are thought to contribute to the disease development. Several cellular abberations in eutopic endometrium and lesions of women with endometriosis are already known to play essential roles in the establishment and maintenance of endometriotic lesions. Proliferation, migration and adhesion are altered in cells from patients with endometriosis compared to control patients. The aim of this project is to evaluate closer molecular/cellular aberrations within the development and course of endometriosis.  Furthermore, we attempt to search for stable changes in factors involved in the process of endometriosis in different bio-fluids in order to identify potential candidates for non-invasive disease diagnosis and monitoring. We will investigate different expression levels of proteins using ELISA, PCR and western blot techniques. Additionally, statistic and bioinformatic modeling will be done in order to identify the most promising targets.

Methods and Skills:

Cell culture; ELISA; Western blot

Publications:

 Yotova I, Quan P, Gaba A, Leditznig N, Pateisky P, Kurz C, Tschugguel W. Raf-1 levels determine the migration rate of primary endometrial stromal cells of patients with endometriosis. J Cell Mol Med 16: 2127-2139,2012 

Kuessel L, Jaeger-Lansky A, Pateisky P, Rossberg N, Schulz A, Schmitz AA, Staudigl C, Wenzl R. Cytokeratin-19 as a biomarker in urine and in serum for the diagnosis of endometriosis-a prospective study. Gynecol Endocrinol. 2014 Jan;30(1):38-41. Epub 2013 Nov 12.