The Competence Center Oral Biology is involved in dental research and training. The work is done in an interdisciplinary team with national and international cooperation, integrating academia and industry. The laboratory uses fundamental cell biology and preclinical models for the development of causal therapeutic strategies in regenerative dentistry. Gruber and his interdisciplinary group focus on bone regeneration with respect to osseointegration of dental implants and the consolidation of grafts and biomaterials, with particular emphasis on implant dentistry and periodontology. His expertise lies on the molecular and cellular mechanisms of oral wound healing and bone regeneration in health and disease. Gruber and his team have developed a strong research profile to reveal the clinical relevance of natural and synthetic molecules and biological fluids in dental research. They have published so far over 200 original articles (Scopus ID: 7102883530; orcid.org/0000-0001-5400-9009).
Methods & Techniques
The research portfolio includes fundamental in vitro cell culture techniques with a focus on oral and bone cells such as periodontal fibroblasts, osteoblasts and osteoclasts. Based on a broad range of methods for the determination of proliferation, migration, differentiation, signalling and gene expression, we test the reaction of cells to various biomaterials, bone transplants, saliva and bioactive molecules of natural origin. The portfolio further includes various preclinical models with a focus on bone regeneration and histological and µCT analysis.
Di Summa F, Kargarpour Z, Nasirzade J, Stähli A, Mitulović G, Panić-Janković T, Koller V, Kaltenbach C, Müller H, Panahipour L, Gruber R, Strauss FJ: TGFβ activity released from platelet-rich fibrin adsorbs to titanium surface and collagen membranes. Scientific Reports 10:10203, 2020
Dobsak T, Heimel P, Tangl S, Schwarze UY, Schett G, Gruber R: Impaired periodontium and temporomandibular joints in tumour necrosis factor-α transgenic mice. Journal of Clinical Periodontology 44: 1226-1235, 2017
Gruber R: Osteoimmunology: Inflammatory osteolysis and regeneration of the alveolar bone. Journal of Clinical Periodontology 46 (Suppl 21): 52-69, 2019
Kuchler U, Heimel P, Stähli A, Strauss FJ, Luza B, Gruber R: Impact of DBBM fragments on the porosity of the calvarial bone: A Pilot Study on Mice. Materials (Basel) 13: 4748, 2020
Lee JS, Mitulović G, Panahipour L, Gruber R: Proteomic analysis of porcine-derived collagen membrane and matrix. Materials (Basel) 13: 5187, 2020
Panahipour L, Kargarpour Z, Laggner M, Mildner M, Ankersmit HJ, Gruber R: TGF-β in the secretome of irradiated peripheral blood mononuclear cells supports in vitro osteoclastogenesis. International Jouranl of Molecular Sciences 21: 8569, 2020
Schwarze UY, Strauss FJ, Gruber R: Caspase inhibitor attenuates the shape changes in the alveolar ridge following tooth extraction: A pilot study in rats. Journal of Periodontal Research 2020 [Online ahead of print]
Strauss FJ, Stähli A, Beer L, Mitulović G, Gilmozzi V, Haspel N, Schwab G, Gruber R: Acid bone lysate activates TGFβ signalling in human oral fibroblasts. Scientific Reports 8: 16065, 2018
Strauss FJ, Stähli A, Kobatake R, Tangl S, Heimel P, Apaza Alccayhuaman KA, Schosserer M, Hackl M, Grillari J, Gruber R. miRNA-21 deficiency impairs alveolar socket healing in mice. Journal of Periodontology 91: 1664-1672, 2020