OEHLER Rudolf, Ph.D.

Research Interests: Immunologic Response to Cell Death

Our research interest is to characterize the immunologic response to therapy-induced cell death. For example, during chemotherapy massive cell death occurs and the immune system is confronted with lots cell debris but is also exposed to inhibitory properties of the drug. We investigate which mechanisms are responsible for the elimination of the cell debris and how it can be enhanced. Special interest is given to the role of complement components in this context. We could show that these mechanisms play a role in the response to chemotherapy of mamma carcinoma patients and that their plasma components can be used as predictive biomarkers.

Methods & Techniques

Basic molecular and cellular biology techniques; „State of the art“ proteom analysis by 2D-DIGE technology; fluorescence-based quantitative 1D and 2D Western blotting; primary cell culture; flow cytometry; in vitro functional cell assays

Selected Publications

Veitinger M, Umlauf E, Baumgartner R, Badrnya S, Porter J, Lamont J, Gerner C, Gruber CW, Oehler R, Zellner M. A combined proteomic and genetic analysis of the highly variable platelet proteome: From plasmatic proteins and SNPs. Journal of Proteomics 75: 5848-5860, 2012

Bilalic S, Michlmayr A, Gruber V, Buchberger E, Burghuber C, Böhmig GA, Oehler R. Lymphocyte activation induces cell surface expression of an immunogenic vimentin isoform. Transplant Immunology 27: 101-106, 2012

Zellner M, Graf A, Zehetmayer S, Winkler W, Staes A, Gevaert K, Vandekerckhove J, Marchetti-Deschmann M, Miller I, Bauer P, Allmaier G, Oehler R. How many spots with missing values can be tolerated in quantitative two-dimensional gel electrophoresis when applying univariate statistics? Journal of Proteomics 75: 1792-1802, 2012

Graf A, Oehler R. Study design in DIGE-based biomarker discovery. Methods in Molecular Biology 854: 195-206, 2012

Michlmayr A, Bachleitner-Hofmann T, Baumann S, Marchetti-Deschmann M, Rech-Weichselbraun I, Burghuber C, Pluschnig U, Bartsch R, Graf A, Greil R, Allmaier G, Steger , Gnant, M, Bergmann M, Oehler R. Modulation of plasma complement by the initial dose of epirubicin/docetaxel therapy in breast cancer and its predictive value. British Journal of Cancer 103: 1201-1208, 2010

Bilalic S, Veitinger M, Ahrer K-H, Gruber V, Zellner M, Brostjan C, Bartel G, Cejka D, Reichel C, Jordan V, Burghuber C, Mühlbacher F, Böhmig GA, Oehler R. Identification of non-HLA antigens targeted by alloreactive antibodies in patients undergoing chronic hemodialysis. Journal of Proteome Research 9: 1041-1049, 2010

Winkler W, Zellner M, Diestinger M, Babeluk R, Marchetti M, Goll A, Zehetmayer S, Bauer P, Rappold E, Miller I, Roth E, Allmaier G, Oehler R. Biological variation of the platelet proteome in the elderly population and its implication for biomarker research. Molecular and Cellular Proteomics 7: 193-203, 2008