Detail

Keywords

Animal models; Dermatitis, Atopic; Flow Cytometry; Inflammation; Microscopy, Confocal

Research group(s)

• Tissue Homeostasis Group
  Head: Karin Pfisterer
  Members:
  Pauline Weinzettl
  David Samardzic
  Parvaneh Balsini
  Karin Pfisterer

Research interests

My main research focus is to understand immediate and delayed immune responses upon hapten sensitization of mouse skin as a model for allergen entry. We will use an atopic dermatitis (AD) mouse model to mimic allergen entry and monitor immune cell infiltration and migration in the skin in real-time. Further, we would like to specifically investigate the contribution of T cells and their subsets in AD using imaging techniques such as confocal microscopy, intravital multiphoton microscopy (MP-IVM), and flow cytometry.

Techniques, methods & infrastructure

• Mouse model 
• Immunofluorescence
• Intravital multiphoton microscopy 
• Confocal microscopy
• Flow cytometry
• Cell culture
• qPCR

Selected publications

1. Koleini, F., Balsini, P. and Parastar, H. (2020) ‘Evaluation of partial least-squares regression with multivariate analytical figures of merit for determination of 10 pesticides in milk’, International Journal of Environmental Analytical Chemistry, 102(8), pp. 1900–1910. Available at: http://dx.doi.org/10.1080/03067319.2020.1745198.
2. Balsini, P. and Parastar, H. (2019) ‘Development of multi‐response optimization and quadratic calibration curve for determination of ten pesticides in complex sample matrices using QuEChERS dispersive liquid–liquid microextraction followed by gas chromatography’, Journal of Separation Science, 42(23), pp. 3553–3562. Available at: http://dx.doi.org/10.1002/jssc.201900823.