Mag. med. vet. Johannes Pfabe
Center for Physiology and Pharmacology (Institute of Physiology)
Position: PHD Student
johannes.pfabe@meduniwien.ac.at
Keywords
Calcium; Diabetes Mellitus; Pancreas; Patch-Clamp Techniques; Ryanodine Receptor Calcium Release Channel; Time-Lapse Imaging
Research group(s)
- Cell and tissue networks
Head: Marjan Slak Rupnik
Research Area: Collective activity presents a basic mode of operation in complex cell and tissue network activity and can be assessed using various advanced computational tools.
Members:
Techniques, methods & infrastructure
- Acute pancreas tissue slices from mice and human
- Confocal time-lapse imaging of [Ca2+] in acute pancreatic tissue slices
- Patch-clamp measurements in acute pancreatic tissue slices
- Immunofluorescence microscopy
- Insulin assay
- Modern data analysis
Selected publications
- Postić, S. et al. (2022) ‘pH-Dependence of Glucose-Dependent Activity of Beta Cell Networks in Acute Mouse Pancreatic Tissue Slice’, Frontiers in Endocrinology, 13. Available at: http://dx.doi.org/10.3389/fendo.2022.916688.
- Postić, S. et al. (2021) ‘High resolution analysis of the cytosolic Ca2+ events in beta cell collectives in situ’. Available at: http://dx.doi.org/10.1101/2021.04.14.439796.