Detail

Keywords

Biomimetic Materials; Nerve Regeneration; Peripheral Nerves; Regenerative Medicine; Schwann Cells; Silk

Research group(s)

• ARGE Radtke
  Research Area: cell based therapies and spider silk conduits for peripheral nerve regeneration
  Members:
  Aida Naghilou
  Maximilian Haertinger
  Tamara Weiss
  Sarah Isabella Stadlmayr
  Paul Supper

Research interests

Within the scope of my research, I try to unravel the unique properties of spider silk responsible for its exceptional ability to support peripheral nerve regeneration. I combine methods such as mass spectrometry, electron microscopy, and atomic force microscopy, with in vitro studies to determine the crucial chemical, structural, and morphological properties of spider silk.

Techniques, methods & infrastructure

• Primary Rodent Cell Culture
• Live Cell Imaging
• Confocal Laser Scanning Microscopy
• Atomic Force Microscopy
• Nanoindentation
• Electron Microscopy 
• Proteomics (nLC-nESI-MS/MS)
• Transcriptomics (RNASeq, Microarray)
• Zeta Potential Measurements

Selected publications

1. Stadlmayr, S. et al. (2023) ‘Comparative Analysis of Various Spider Silks in Regard to Nerve Regeneration: Material Properties and Schwann Cell Response’, Advanced Healthcare Materials [Preprint]. Available at: https://doi.org/10.1002/adhm.202302968..
2. Bergmann, F. et al. (2022) ‘The properties of native Trichonephila dragline silk and its biomedical applications’, Biomaterials Advances, 140, p. 213089. Available at: http://dx.doi.org/10.1016/j.bioadv.2022.213089.
3. Semmler, L. et al. (2023) ‘Silk‐in‐Silk Nerve Guidance Conduits Enhance Regeneration in a Rat Sciatic Nerve Injury Model’, Advanced Healthcare Materials, p. 2203237. Available at: http://dx.doi.org/10.1002/adhm.202203237.
4. Millesi, F. et al. (2023) ‘Systematic Comparison of Commercial Hydrogels Revealed That a Synergy of Laminin and Strain-Stiffening Promotes Directed Migration of Neural Cells’, ACS Applied Materials & Interfaces, 15(10), pp. 12678–12695. Available at: http://dx.doi.org/10.1021/acsami.2c20040.
5. Naghilou, A. et al. (2023) ‘Insights into the material properties of dragline spider silk affecting Schwann cell migration’, International Journal of Biological Macromolecules, 244, p. 125398. Available at: https://doi.org/10.1016/j.ijbiomac.2023.125398. Available at: http://dx.doi.org/.