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Detail

Christian  Pifl
Ao.Prof. Dr. Christian Pifl

Center for Brain Research (Division of Molecular Neurosciences)
Position: Associate Professor

T +43 1 40160 34080
christian.pifl@meduniwien.ac.at

Keywords

Catecholamine Plasma Membrane Transport Proteins; Neurochemistry; Neuropharmacology; Parkinson Disease

Techniques, methods & infrastructure

  • HPLC with electrocehmical detecion for quantitative determination of noradrenaline, dopamine, 3,4-dihydroxyphenyl acetic acid, homovanillic acid, serotonin, 5-hydroxyindol acetic acid.
  • HPLC with fluorometric detection after precolumn derivatization for quantitative determination of aspartate, glutamate, glutamine, asparagine, glycine, serine, alanine, threonine, taurine, GABA.
  • Radioligand binding assays, transport and release (superfusion) assays on synaptosomes, synaptic vesicles, transfected cells.
  • Radiochemical assays for glutamic acid decarboxylase, choline acetyl transferase.

Selected publications

  1. Pifl C, Rajput A, Reither H, Blesa J, Cavada C, Obeso JA, Rajput AH, Hornykiewicz O (2014) Is Parkinson's Disease a Vesicular Dopamine Storage Disorder? Evidence from a Study in Isolated Synaptic Vesicles of Human and Nonhuman Primate Striatum J Neurosci, 24: 8210-8218
  2. Pifl C, Stephen J, Kish SJ, Hornykiewicz O (2012) Thalamic noradrenaline in Parkinson's disease: Deficits suggest role in motor and non-motor symptoms Movement Disorders, 27: 1618-1624
  3. Peneder TM, Scholze P, Berger ML, Reither H, Heinze G, Bertl J, Bauer J, Richfield EK, Hornykiewicz O, Pifl C (2011) Chronic exposure to manganese decreases striatal dopamine turnover in human alpha-synuclein transgenic mice Neuroscience, 180: 280-292
  4. Pifl C, Singer EA (1999) Ion dependence of carrier-mediated release in dopamine or norepinephrine transporter-transfected cells questions the hypothesis of faciliated exchange diffusion Mol Pharmacol, 56: 1047-1054
  5. Sitte HH, Huck S, Reither H, Boehm S, Singer EA, Pifl C (1998) Carrier-mediated release, transport rates and charge transfer induced by amphetamine, tyramine and dopamine in mammalian cells transfected with the human dopamine transporter J Neurochem, 71: 1289-1297