Detail

Keywords

Biochemistry; Endothelium, Vascular; Flow Cytometry; Fluorescence; Fluorescence Recovery After Photobleaching; Fluorescence Resonance Energy Transfer; Inflammation; Mice, Transgenic; Microscopy; Molecular Biology; NF-kappa B; Oncology; Signal Transduction; Thrombosis; Transcription Factors

Research group(s)

• JohannesSchmid-Lab
  Head: Johannes A. Schmid
  Research Area: Inflammation - vascular biology - thrombosis - cancer - NF-kappa B
  Members:
  Johannes A. Schmid

Research interests

Current research interests focus on inflammation, thrombosis and cancer. Signal transduction pathways of inflammation are studied with special focus on the NF-kappa B signaling, as well as interconnections with other signaling processes in vascular biology and cancer .   Signal transduction networks are studied with a variety of experimental systems, such as cell culture of primary and transformed cells or transgene mouse models. Transfection and viral transduction strategies are applied to achieve either ectopic expression or gene suppression of effector molecules followed by analyzing a variety of biological readouts such as cell proliferation, apoptosis or activation of cells.

Techniques, methods & infrastructure

• epifluorescence microscopy (of life cells)
• laser scanning microscopy including FLIP and FRAP microscopy
• FRET (Fluorescence Resonance Energy Transfer) measurements and microscopy
• DNA-protein binding assays (EMSA's and non-radioactive alternatives)
• protein-protein interaction assays (co-immunoprecipitation, FRET, yeast 2-hybrid, mammalian 2-hybrid etc.)
• reporter gene assays
• flow cytometry and cell sorting
• tissue cytometry
• histology
• kinase assays
• realtime PCRs
• Northern Blots
• molecular biology and cloning
• biochemistry methods
• cell biology methods
• transfections
• RNA interference
• radioactive labeling
• ultra-centrifugation methods (subcellular fractionation)
• fluorometry
• chromatography
• in situ hybridizations
• cell cycle profiling
• apoptosis assays
• proliferation assays
• in vitro fusion of cellular compartments
• concepts of genome editing (CRISPR/Cas9)

Grants

• KI-Blutzellanalyse (Multifactorial fluorescence microscopy and AI-assisted analysis of blood smears) (2022)
  Source of Funding: FFG (Austrian Research Promotion Agency), BRIDGE
  Coordinator of the collaborative project
• Genomische und zelluläre Effekte der Wechselwirkung zwischen einem Entzündungsmolekül und einem Onkogen (2021)
  Source of Funding: Medical Scientific Fund of the Mayor of the City of Vienna, Krebsforschungsfonds
  Principal Investigator
• Histology 2.0 (2019)
  Source of Funding: FFG (Austrian Research Promotion Agency), BRIDGE-programme
  Principal Investigator
• Dynamics of molecular interactions in live cells (2015)
  Source of Funding: FWF (Austrian Science Fund), Stand-Alone Projects
  Principal Investigator
• Cellular Mediators Linking Inflammation and Thrombosis (2014)
  Source of Funding: FWF (Austrian Science Fund), Special Research Program (Spezialforschungsbereich)
  Coordinator of the collaborative project
• Transcription Factors in Inflammation and Cancer (2011)
  Source of Funding: FWF (Austrian Science Fund), Stand-Alone Projects
  Principal Investigator

Selected publications

1. Hoesel, B. & Schmid, J.A., 2013. The complexity of NF-κB signaling in inflammation and cancer. Mol Cancer, 12(1), p.86. Available at: http://dx.doi.org/10.1186/1476-4598-12-86.
2. Neoplasia. 2011 Aug;13(8):692-703. Persistent inflammation leads to proliferative neoplasia and loss of smooth muscle cells in a prostate tumor model. Birbach A, Eisenbarth D, Kozakowski N, Ladenhauf E, Schmidt-Supprian M, Schmid JA.
3. Sughra, K. et al., 2010. Interaction of the TNFR-Receptor Associated Factor TRAF1 with I-Kappa B Kinase-2 and TRAF2 Indicates a Regulatory Function for NF-Kappa B Signaling S. Agarwal, ed. PLoS ONE, 5(9), p.e12683. Available at: http://dx.doi.org/10.1371/journal.pone.0012683.
4. Schmid, J.A. & Birbach, A., 2008. IκB kinase β (IKKβ/IKK2/IKBKB) A key molecule in signaling to the transcription factor NF-κB. Cytokine & Growth Factor Reviews, 19(2), pp.157-165. Available at: http://dx.doi.org/10.1016/j.cytogfr.2008.01.006.
5. Orel, L. et al., 2009. Crosstalk between the NF-κB activating IKK-complex and the CSN signalosome. Journal of Cellular and Molecular Medicine, 14(6b), pp.1555-1568. Available at: http://dx.doi.org/10.1111/j.1582-4934.2009.00866.x.