AF021: Subtilisin-like serine protease

List allergens from this family:

Routes of exposure:
Include only IUIS approved allergens.

Biochemical properties

The subtilisin family is the second largest serine protease family characterized to date [1]. Its members are found in archaea, bacteria, eukaryotes, and viruses. Structures have been determined for several members of the subtilisin family. They contain the same catalytic triad as the chymotrypsins, although the residues occur in a different order. Apart from this, the structures show no other similarities.

Allergens from this family

Most allergens from this family are fungal allergens. They belong to the subfamilies of alkaline (Asp f 13 from Aspergillus fumigatus and Pen c 1 from Penicillium citrinum) or vacuolar serine proteases (Asp f 18 and Pen c 18) [2]. The only plant allergens known to belong to this family is Cuc m 1 from muskmelon [3].



  1. Siezen RJ, Leunissen JA:
    Subtilases: the superfamily of subtilisin-like serine proteases.
    Protein Sci 1997, 6, 501-23. [PubMed] [Full Text]
  2. Shen HD, Tam MF, Chou H, Han SH:
    The importance of serine proteinases as aeroallergens associated with asthma.
    Int Arch Allergy Immunol 1999, 119, 259-64. [PubMed] [Full Text]
  3. Cuesta-Herranz J, Pastor C, Figueredo E, Vidarte L, De las Heras M, Duran C, Fernandez-Caldas E, de Miguel J, Vivanco F:
    Identification of Cucumisin (Cuc m 1), a subtilisin-like endopeptidase, as the major allergen of melon fruit.
    Clin Exp Allergy 2003, 33, 827-33. [PubMed]


Links to Pfam

Family-defining Pfam domains (at least one of these domains is present in each family member):

Pfam domain Pfam clan
PF00082 Subtilase family -
PF05922 Peptidase inhibitor I9 CL0570 Protease propeptides/inhibitors

Links to Wikipedia


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