AF033: Alpha-amylase

List allergens from this family:

Routes of exposure:
Include only IUIS approved allergens.

Biochemical properties

Most members of the glycoside hydrolase family 13 are α-amylases. The maltogenic α-amylase is an enzyme which catalyzes hydrolysis of 1,4-α-D-glucosidic linkages in polysaccharides so as to remove successive α-maltose residues from the non-reducing ends of the chains. The catalytic domain has a structure consisting of an eight-stranded α/β barrel that contains the active site, interrupted by a 70 amino acid calcium-binding domain protruding between β-strand 3 and α-helix 3, and a carboxyl-terminal Greek key β-barrel domain [1].

Allergens from this family

Allergens from this family were identified in diverse organisms. The group 4 mite and group 11 cockroach allergens are minor allergens binding IgE from about 40% of mite or cockroach allergic patients' sera [2, 3]. Cereal α-amylases are important allergens for patients with baker's asthma [4]. Some of these patients also show IgE reactivity to fungal α-amylases used as baking additives or present in mold contaminated flour [5].



  1. van der Maarel MJ, van der Veen B, Uitdehaag JC, Leemhuis H, Dijkhuizen L:
    Properties and applications of starch-converting enzymes of the alpha-amylase family.
    J Biotechnol 2002, 94, 137-55. [PubMed]
  2. Thomas WR, Smith WA, Hales BJ, Mills KL, O'Brien RM:
    Characterization and immunobiology of house dust mite allergens.
    Int Arch Allergy Immunol 2002, 129, 1-18. [PubMed] [Full Text]
  3. Jeong KY, Kim CR, Park J, Han IS, Park JW, Yong TS:
    Identification of novel allergenic components from German cockroach fecal extract by a proteomic approach.
    Int Arch Allergy Immunol 2013, 161, 315-24. [PubMed] [Full Text]
  4. Sandiford CP, Tee RD, Taylor AJ:
    The role of cereal and fungal amylases in cereal flour hypersensitivity.
    Clin Exp Allergy 1994, 24, 549-57. [PubMed]
  5. Baur X, Chen Z, Sander I:
    Isolation and denomination of an important allergen in baking additives: alpha-amylase from Aspergillus oryzae (Asp o II).
    Clin Exp Allergy 1994, 24, 465-70. [PubMed]


Links to Pfam

Family-defining Pfam domains (at least one of these domains is present in each family member):

Pfam domain Pfam clan
PF00128 Alpha amylase, catalytic domain CL0058 Tim barrel glycosyl hydrolase superfamily
PF02806 Alpha amylase, C-terminal all-beta domain CL0369 Glycosyl hydrolase domain superfamily

Additional Pfam domains found in some family members:

Pfam domain Pfam clan
PF09260 Domain of unknown function (DUF1966) CL0369 Glycosyl hydrolase domain superfamily

Links to Wikipedia


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