AF052: Glycoside hydrolase family 32

List allergens from this family:

Routes of exposure:
Include only IUIS approved allergens.

Biochemical properties

This family contains enzymes with a wide range of activities comprising hydrolases such as invertase, inulinase, and other polysaccharide hydrolases as well as transferases such as sucrose:sucrose 1-fructosyl transferase. Family 32 glycosyl hydrolases comprise two distinct domains. The N-terminal domain, which forms a five bladed beta propeller, and the C-terminal domain, which forms a β-sandwich structure [1].

Allergens from this family

Sola l 2 (previously Lyc e 2), a β-fructofuranosidase from tomato, shows IgE reactivity due to its carbohydrate determinants [2].



  1. Alberto F, Bignon C, Sulzenbacher G, Henrissat B, Czjzek M:
    The three-dimensional structure of invertase (beta-fructosidase) from Thermotoga maritima reveals a bimodular arrangement and an evolutionary relationship between retaining and inverting glycosidases.
    J Biol Chem 2004, 279, 18903-10. [PubMed] [Full Text]
  2. Westphal S, Kolarich D, Foetisch K, Lauer I, Altmann F, Conti A, Crespo JF, Rodriguez J, Enrique E, Vieths S, Scheurer S:
    Molecular characterization and allergenic activity of Lyc e 2 (beta-fructofuranosidase), a glycosylated allergen of tomato.
    Eur J Biochem 2003, 270, 1327-37. [PubMed]


Links to Pfam

Family-defining Pfam domains (at least one of these domains is present in each family member):

Pfam domain Pfam clan
PF00251 Glycosyl hydrolases family 32 N-terminal domain CL0143 Beta fructosidase superfamily
PF08244 Glycosyl hydrolases family 32 C terminal CL0004 Concanavalin-like lectin/glucanase superfamily

Additional Pfam domains found in some family members:

Pfam domain Pfam clan
PF11837 Domain of unknown function (DUF3357) -

Links to Wikipedia


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