AF063: Beta-1,3-glucanase and X8 domain

List allergens from this family:

Routes of exposure:
Include only IUIS approved allergens.

Biochemical properties

Glycoside hydrolase family 17 comprises enzymes from plants and fungi with several known activities: endo-1,3-β-glucosidase, lichenase, and exo-1,3-glucanase. They fold into an (αβ)8 TIM barrel structure. Some glucanases contain on their C-terminus an X8 domain (Pfam:PF07983), a small domain that contains 6 conserved cysteine residues that presumably form three disulphide bridges [1]. Two X8-family members from Arabidopsis thaliana were identified as callose-binding plasmodesma proteins that provide an anchor between the plasma membrane and the cell wall [2].

Allergens from this family

β-1,3-Glucanases from plants were described as allergens in Hevea brasiliensis latex [3], olive pollen [4] and plant foods [5]. They are glycoproteins that contain IgE-binding N-linked glycans and are thought to be involved in the latex-fruit syndrome. Olive pollen glucanase, Ole e 9, contains an X8 domain at its C-terminus. Ole e 10 consists only of an X8-domain, lacking a glucanase domain [6].



  1. Henrissat B, Davies GJ:
    Glycoside hydrolases and glycosyltransferases. Families, modules, and implications for genomics.
    Plant Physiol 2000, 124, 1515-9. [PubMed]
  2. Simpson C, Thomas C, Findlay K, Bayer E, Maule AJ:
    An Arabidopsis GPI-anchor plasmodesmal neck protein with callose binding activity and potential to regulate cell-to-cell trafficking.
    Plant Cell 2009, 21, 581-94. [PubMed] [Full Text]
  3. Yagami T, Osuna H, Kouno M, Haishima Y, Nakamura A, Ikezawa Z:
    Significance of carbohydrate epitopes in a latex allergen with beta-1,3-glucanase activity.
    Int Arch Allergy Immunol 2002, 129, 27-37. [PubMed] [Full Text]
  4. Palomares O, Villalba M, Quiralte J, Polo F, Rodriguez R:
    1,3-beta-glucanases as candidates in latex-pollen-vegetable food cross-reactivity.
    Clin Exp Allergy 2005, 35, 345-51. [PubMed] [Full Text]
  5. Wagner S, Radauer C, Hafner C, Fuchs H, Jensen-Jarolim E, Wuthrich B, Scheiner O, Breiteneder H:
    Characterization of cross-reactive bell pepper allergens involved in the latex-fruit syndrome.
    Clin Exp Allergy 2004, 34, 1739-46. [PubMed] [Full Text]
  6. Barral P, Batanero E, Palomares O, Quiralte J, Villalba M, Rodriguez R:
    A major allergen from pollen defines a novel family of plant proteins and shows intra- and interspecies [correction of interspecie] cross-reactivity.
    J Immunol 2004, 172, 3644-51. [PubMed]


Links to Pfam

Family-defining Pfam domains (at least one of these domains is present in each family member):

Pfam domain Pfam clan
PF00332 Glycosyl hydrolases family 17 CL0058 Tim barrel glycosyl hydrolase superfamily
PF07983 X8 domain -

Links to Wikipedia


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