Research Group of Johannes A. Schmid

• Protocols: mobile phone version

•  DNA-constructs (Google spreadsheet)

• Addgene plasmids

•  Links to plasmids with maps (powered by GenomeCompiler)

•  Research Material (plasmids, primers, cells)

•  Molarity Calculator

• Macros for ImageJ

Curve fitting with R: Example: R-Markdown-link

MS-Excel Templates                         

•  New: dose-response curve (with curve fitting and R2)

• DNA-calculations

• Biorad Protein Assay

•  BCA-Protein Assay (Harald Freudenthaler)

•  FLIP (fluorescence loss in photobleaching)

•  FRAP (fluorescence recovery after photobleaching)               
  - template for single values
  - template for 5 replicates + curve fitting ....NEW
    (Solver Add-In has to be activated)
  - template for 5 replicates
  - template for single values with control region

•  FRET: correction factors, Youvan and Xia values

•  Molarity calculations

•  realtime PCR (quantitative PCR, qPCR: Link to Online Excel)
  - with dilution curve to determine PCR effciency

•  realtime PCR with quantification of the PCR efficiency based on the amplification curve

•  SDS-PAGE: MW-calculations

Some Selected Protocols 

• Collection of standard methods
  (from Hannah Neumeier's diploma thesis)

•  beta-Galactosidase CPRG plate reader assay

•  Cell cycle analysis by PI-staining and flow cytometry 

•  Co-immunoprecipitation

•  EMSA's (electrophoretic mobility shift assays)

•  ISNT (In Situ Nick Translation for the morphological detection of apoptotic cells)

•  JC1-staining to detect apoptotic cells (via the mitochondrial membrane potential)

•  Kinase assays

•  Northern Blots

•  PCR-cloning

•  SDS-PAGE buffer recipes

•  Transfections with Calcium

•  Transfections with Lipofectamine Plus

•  Western Blots
  (for detection of myc, HA, flag, I-kappa B, GFP, TAK1)

•  Yeast-DNA preparation

•  Yeast transformation (link to Gietz lab)
  

Links to some of our research material:

•  DNA-constructs

•  Primers

•  Cell lines and stable transfectants

Link to the Belgian collection of plasmids and microorganisms

Some other Links:

•  FRET efficiency > distance calculator: