Detail

Keywords

Biomimetic Materials; Nerve Regeneration; Peripheral Nerves; Regenerative Medicine; Schwann Cells; Silk

Research group(s)

• ARGE Radtke
  Research Area: cell based therapies and spider silk conduits for peripheral nerve regeneration
  Members:
  Paul Supper
  Aida Naghilou
  Maximilian Haertinger
  Tamara Weiss
  Sarah Isabella Stadlmayr

Research interests

Within the scope of my research, I try to unravel the unique properties of spider silk responsible for its exceptional ability to support peripheral nerve regeneration. I combine methods such as mass spectrometry, electron microscopy, and atomic force microscopy, with in vitro studies to determine the crucial chemical, structural, and morphological properties of spider silk.

Techniques, methods & infrastructure

• Primary Rodent Cell Culture
• Live Cell Imaging
• Confocal Laser Scanning Microscopy
• Atomic Force Microscopy
• Nanoindentation
• Electron Microscopy 
• Proteomics (nLC-nESI-MS/MS)
• Transcriptomics (RNASeq, Microarray)

Selected publications

1. Borger, A. et al. (2022) ‘How miRNAs Regulate Schwann Cells during Peripheral Nerve Regeneration—A Systemic Review’, International Journal of Molecular Sciences, 23(7), p. 3440. Available at: http://dx.doi.org/10.3390/ijms23073440.
2. Bergmann, F. et al. (2022) ‘The properties of native Trichonephila dragline silk and its biomedical applications’, Biomaterials Advances, 140, p. 213089. Available at: http://dx.doi.org/10.1016/j.bioadv.2022.213089.
3. Semmler, L. et al. (2023) ‘Silk‐in‐Silk Nerve Guidance Conduits Enhance Regeneration in a Rat Sciatic Nerve Injury Model’, Advanced Healthcare Materials, p. 2203237. Available at: http://dx.doi.org/10.1002/adhm.202203237.
4. Millesi, F. et al. (2023) ‘Systematic Comparison of Commercial Hydrogels Revealed That a Synergy of Laminin and Strain-Stiffening Promotes Directed Migration of Neural Cells’, ACS Applied Materials & Interfaces, 15(10), pp. 12678–12695. Available at: http://dx.doi.org/10.1021/acsami.2c20040.
5. Naghilou, A. et al. (2023) ‘Insights into the material properties of dragline spider silk affecting Schwann cell migration’, International Journal of Biological Macromolecules, 244, p. 125398. Available at: https://doi.org/10.1016/j.ijbiomac.2023.125398. Available at: http://dx.doi.org/.