Detail

Keywords

Biomarkers, Pharmacological; Epithelial-Mesenchymal Transition; Neoplasm Metastasis; Signal Transduction

Research group(s)

• Tumor Progression and Metastasis
  Members:
  Wolfgang Mikulits

Research interests

• Mechanisms of epithelial to mesenchymal transition and liver carcinoma progression
• Tumor-promoting cell signaling in liver cancer metastasis
• Biomarker of liver cancer
• Models of liver carcinoma progression
• Selective translation control during tumor progression and metastasis

Techniques, methods & infrastructure

• Molecular cloning
• Cell culture of primary cells and cell lines
• 3-dimensional culture
• Real-time qPCR
• RNA immunoprecipitation
• Flow cytometric analysis
• ELISA; Western blot; IHC; Confocal IF
• CRISPR/Cas9; RNA interfence
• Xenograft mouse models
• Syngeneic mouse models
• Invasion and metastasis assays
• Transcriptome profiling
• Proteomic profiling

Grants

• International PhD Program in Translational Oncology (2019)
  Source of Funding: FWF (Austrian Science Fund), doc.funds Programme
  Principal Investigator
• Role of Axl Receptor Shedding in Liver Cancer (2019)
  Source of Funding: FWF (Austrian Science Fund), Stande Alone Project
  Principal Investigator
• Diagnostic and Prognostic Value of Soluble Axl in Hepatocellular Carcinoma (2015)
  Source of Funding: OeNB (Oesterreichische Nationalbank), Anniversary Fund
  Principal Investigator
• Release of Soluble Axl and Gas6-Independent Axl Signaling in Hepatocellular Carcinoma (2015)
  Source of Funding: Herzfelder'sche Familienstiftung,
  Principal Investigator
• Control of Pro- and Anti-Oncogenic Stat3 Activity in Hepatocellular Carcinoma (2012)
  Source of Funding: Herzfelder'sche Familienstiftung, Stand Alone Project
  Principal Investigator
• IT-LIVER - Strategy to Inhibit TGF-beta in Liver Disease (project partner) (2012)
  Source of Funding: EU, FP7-PEOPLE-2012-ITN
  Principal Investigator
• The Role of Axl in Hepatocellular Carcinoma Progression (2012)
  Source of Funding: FWF (Austrian Science Fund), Stand-Alone Projects
  Principal Investigator
• Cooperation of wnt beta-catenin during Hepatocellular Carcinoma Progression (2009)
  Source of Funding: City of Vienna, Hochschuljubiläumsstiftung
  Principal Investigator
• Functional analysis of Laminin B1 IRES in cancer progression (2008)
  Source of Funding: FWF (Austrian Science Fund), Stand-Alone Projects
  Principal Investigator
• Migration and Anoikis during Hepatocellular Carcinoma Progression (2008)
  Source of Funding: City of Vienna, Hochschuljubiläumsstiftung
  Principal Investigator
• RESOLVE Chronic Inflammation and Achieve Healthy Aging by Understanding Non-regenerative Repair (2008)
  Source of Funding: EU, HEALTH-F4-2008-202047
  Principal Investigator
• The Impact of the Tumor Microenvironment in Liver Carcinoma Progression (2008)
  Source of Funding: Herzfelder'sche Familienstiftung, Stand Alone Project
  Principal Investigator
• Liver cancer stemness and metastasis (2007)
  Source of Funding: FWF (Austrian Science Fund), Stand Alone Project
  Principal Investigator
• Jak-Stat - Signalling from Basis to Disease (project part leader) (2005)
  Source of Funding: FWF (Austrian Science Fund), Special Research Programmes (SFB 28)
  Principal Investigator
• Orthotope Transplantation von Hepatozyten zur Untersuchung der Tumor-suppressiven Wirkung von c-Fos während der Lebertumorigenese (2005)
  Source of Funding: City of Vienna, Hochschuljubiläumsstiftung
  Principal Investigator
• Stats in Liver Fibrosis and Late Stage Heptocarcinogenesis (2005)
  Source of Funding: Herzfelder'sche Familienstiftung, Stand Alone Project
  Principal Investigator
• PDGF signaling during hepatocellular tumor (2003)
  Source of Funding: OeNB (Oesterreichische Nationalbank), Anniversary Fund
  Principal Investigator
• Molecular Mechanisms Involved in Hepatocellular Epithelial to Fibroblastoid Conversion (2002)
  Source of Funding: FWF (Austrian Science Fund), Stand Alone Project
  Principal Investigator
• The Induction of Hepatocellular De-differentiation through the Cooperation of Ha-Ras and TGF-beta (2001)
  Source of Funding: Herzfelder'sche Familienstiftung, Stand Alone Project
  Principal Investigator

Selected publications

1. Haider, C. et al. (2019) ‘Transforming Growth Factor‐β and Axl Induce CXCL5 and Neutrophil Recruitment in Hepatocellular Carcinoma’, Hepatology, 69(1), pp. 222–236. Available at: https://doi.org/10.1002/hep.30166.
2. Reichl, P. et al. (2015) ‘Axl activates autocrine transforming growth factor‐β signaling in hepatocellular carcinoma’, Hepatology, 61(3), pp. 930–941. Available at: https://doi.org/10.1002/hep.27492.
3. Schneller, D. et al. (2011) ‘p19ARF/p14ARF controls oncogenic functions of signal transducer and activator of transcription 3 in hepatocellular carcinoma’, Hepatology, 54(1), pp. 164–172. Available at: https://doi.org/10.1002/hep.24329.
4. Fischer, A.N.M. et al. (2006) ‘PDGF essentially links TGF-β signaling to nuclear β-catenin accumulation in hepatocellular carcinoma progression’, Oncogene, 26(23), pp. 3395–3405. Available at: https://doi.org/10.1038/sj.onc.1210121.
5. Wheelock, M. (2002) ‘Faculty Opinions recommendation of Hepatocytes convert to a fibroblastoid phenotype through the cooperation of TGF-beta1 and Ha-Ras: steps towards invasiveness.’, Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature. Faculty Opinions Ltd. Available at: https://doi.org/10.3410/f.1004801.55605.