Cell Biology; Cell Culture Techniques; Regenerative Medicine
My main research focus is to evaluate and characterize the pathways and molecular mechanisms involed in the PRF function which is widely applied clinically for soft and hard tissue regeneration. To have more insight into the regenerative role of PRF, proteomic and genomic profileing of the PRF with the main focus on TGFbeta signaling is conducted. The anti inflammatory and anti osteoclastogenic effect of PRF and the capacity to neutralize the cytotoxic effect of hydrogen peroxide are studied in detail.
Techniques, methods & infrastructure
Murin bone marrow cells are used for in vitro analysis of macrophage polarization and study inflammation. Primary cells from human (gingival fibroblasts) are applied for in vitro analysis of the role of PRF in TGFbeta signaling pathway. A broad spectrum of biochemical, cell biological, and imaging techniques are used.
- Kargarpour Z, Nasirzade J, Panahipour L, Miron RJ and Gruber R (2021). Liquid PRF Reduces the Inflammatory Response and Osteoclastogenesis in Murine Macrophages. Front. Immunol. 12:636427.
- Kargarpour, Z. et al., 2020. Relative Centrifugal Force (RCF; G-Force) Affects the Distribution of TGF-β in PRF Membranes Produced Using Horizontal Centrifugation. International Journal of Molecular Sciences, 21(20), p.7629. Available at: http://dx.doi.org/10.3390/ijms21207629.
- Kargarpour, Z. et al., 2020. Platelet-Rich Fibrin Can Neutralize Hydrogen Peroxide-Induced Cell Death in Gingival Fibroblasts. Antioxidants, 9(6), p.560. Available at: http://dx.doi.org/10.3390/antiox9060560.
- Kargarpour, Z. et al., 2020. Liquid Platelet-Rich Fibrin and Heat-Coagulated Albumin Gel: Bioassays for TGF-β Activity. Materials, 13(16), p.3466. Available at: http://dx.doi.org/10.3390/ma13163466.
- Kargarpour, Z. et al., 2020. Platelet‐rich fibrin suppresses in vitro osteoclastogenesis. Journal of Periodontology, 91(3), pp.413–421. Available at: http://dx.doi.org/10.1002/JPER.19-0109.