Epithelioid Cells; Granuloma; Macrophages
My research focuses on how macrophages form phenotypically and metabolically distinct granulomas via an 'epithelioid' differentiation stage. I am investigating differences between 'sterile' granulomas such as those seen in sarcoidosis and those induced by infection with the parasitic fluke Schistosoma mansoni. I am also interested in discovering granuloma-inducing pathways and means of inhibiting them through pharmaceutics.
Techniques, methods & infrastructure
In vivo and ex vivo work with transgenic mouse models including surgical procedures. Cell culture, activation assays, cell aggregation assays and visual analysis pipelines, multicolor flow cytometry, immunofluorescence.
- Wilson, J.L., Mayr, H.K. & Weichhart, T., 2019. Metabolic Programming of Macrophages: Implications in the Pathogenesis of Granulomatous Disease. Frontiers in Immunology, 10. Available at: http://dx.doi.org/10.3389/fimmu.2019.02265.
- Wilson, J.L. et al., 2020. Inverse Data-Driven Modeling and Multiomics Analysis Reveals Phgdh as a Metabolic Checkpoint of Macrophage Polarization and Proliferation. Cell Reports, 30(5), pp.1542,