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Detail

Johannes Pfabe
Mag. med. vet. Johannes Pfabe

Center for Physiology and Pharmacology (Institute of Physiology)
Position: PHD Student

johannes.pfabe@meduniwien.ac.at

Keywords

Calcium; Diabetes Mellitus; Pancreas; Patch-Clamp Techniques; Ryanodine Receptor Calcium Release Channel; Time-Lapse Imaging

Research group(s)

Techniques, methods & infrastructure

  • Acute pancreas tissue slices from mice and human
  • Confocal time-lapse imaging of [Ca2+] in acute pancreatic tissue slices
  • Patch-clamp measurements in acute pancreatic tissue slices
  • Immunofluorescence microscopy
  • Insulin assay
  • Modern data analysis

Selected publications

  1. Postić, S. et al. (2022) ‘pH-Dependence of Glucose-Dependent Activity of Beta Cell Networks in Acute Mouse Pancreatic Tissue Slice’, Frontiers in Endocrinology, 13. Available at: http://dx.doi.org/10.3389/fendo.2022.916688.
  2. Postić, S. et al. (2021) ‘High resolution analysis of the cytosolic Ca2+ events in beta cell collectives in situ’. Available at: http://dx.doi.org/10.1101/2021.04.14.439796.