Detail

Keywords

Chemokines; Clinical virology ; Serology

Research group(s)

• Comprehensive Serology and Chemokines in Clinical Virology
  Members:
  Lukas Weseslindtner

Research interests

Serology plays an essential role in the diagnosis of diverse viral diseases. The aim of my research is to evaluate the diagnostic abilities of comprehensive serological assays (avidity assays, epitope type specificity tests, immunoblots, microarrays, surrogate neutralization assays) in clinical virology and to investigate whether T-cell associated chemokines may serve as clinical markers to evaluate the stage, course and severity of different virus infections.

A specific aim of my current research is to assess and evaluate antibody assays in SARS-CoV-2, Parvovirus B19 and Measles virus infections.

Techniques, methods & infrastructure

• ELISAs and CLIAs
• Epitope-Type-Specifity (ETS) Assays
• IgG-avidity assays
• Immunoblots, Microarrays

Grants

• Diagnosis of SARS-CoV-2 infections and assessment of seroprevalence using antibody assays with the highest performance (2020)
  Source of Funding: Medical Scientific Fund of the Mayor of the City of Vienna, CoVID19 Research
  Principal Investigator

Selected publications

1. Semmler, G. et al., 2021. Assessment of S1, S2 and NCP-specific IgM, IgA, and IgG antibody kinetics in acute SARS-CoV-2 infection by a microarray and twelve other immunoassays. Journal of Clinical Microbiology. Available at: http://dx.doi.org/10.1128/JCM.02890-20.
2. Orth-Höller, D. et al., 2021. Kinetics of SARS-CoV-2 specific antibodies (IgM, IgA, IgG) in non-hospitalized patients four months following infection. Journal of Infection, 82(2), pp.282–327. Available at: http://dx.doi.org/10.1016/j.jinf.2020.09.015.
3. Traugott, M.T. et al., 2020. Diagnosis of COVID-19 using multiple antibody assays in two cases with negative PCR results from nasopharyngeal swabs. Infection, 49(1), pp.171–175. Available at: http://dx.doi.org/10.1007/s15010-020-01497-2.
4. Traugott, M. et al., 2020. Performance of Severe Acute Respiratory Syndrome Coronavirus 2 Antibody Assays in Different Stages of Infection: Comparison of Commercial Enzyme-Linked Immunosorbent Assays and Rapid Tests. The Journal of Infectious Diseases, 222(3), pp.362–366. Available at: http://dx.doi.org/10.1093/infdis/jiaa305.
5. Semmler, G. et al., 2020. Elevated CXCL10 Serum Levels in Measles Virus Primary Infection and Reinfection Correlate With the Serological Stage and Hospitalization Status. The Journal of Infectious Diseases, 222(12), pp.2030–2034. Available at: http://dx.doi.org/10.1093/infdis/jiaa326.